製品の概要

  • 製品名
    Anti-IER5 antibody
  • 製品の詳細
    Goat polyclonal to IER5
  • アプリケーション
    適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide:

    C-QPPSGGEDDDAEE

    , corresponding to internal sequence amino acids 248-260 of Human IER5

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

ターゲット情報

  • 関連性
    IER5 is a similar protein to other immediate early response proteins. In mouse, a similar protein may play an important role in mediating the cellular response to mitogenic signals.
  • 参照データベース
  • 別名
    • Immediate early response 5 antibody
    • MGC102760 antibody
    • SBBI48 antibody

画像

  • All lanes : Anti-IER5 antibody (ab59133)

    Lane 1 : Human H1299 cell lysate transfected with empty vector
    Lane 2 : Human H1299 cells lysate overexpressing IER5

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated rabbit polyclonal to goat IgG at 1/1000 dilution

    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

プロトコール

参考文献

ab59133 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (H1299 cells overexpressing IER5)
Loading amount
10 µg
Specification
H1299 cells overexpressing IER5
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Blocking One (Nakalai) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Jul 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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