Anti-Iduronate 2 sulfatase 抗体 (ab85701)

製品の概要

  • 製品名Anti-Iduronate 2 sulfatase antibody
    Iduronate 2 sulfatase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Iduronate 2 sulfatase
  • 特異性Expected to recognize isoform A (NP_000193.1).
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    KHFRFRDLEEDP

    by a Cysteine residue linker, corresponding to internal sequence amino acids 440-451 of Human Iduronate 2 sulfatase (NP_000193.1)

  • ポジティブ・コントロール
    • Human Liver lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

ターゲット情報

  • 機能Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.
  • 組織特異性Liver, kidney, lung, and placenta.
  • 関連疾患Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2) [MIM:309900]; also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation.
  • 配列類似性Belongs to the sulfatase family.
  • 翻訳後修飾The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • 細胞内局在Lysosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha L iduronate sulfate sulfatase antibody
    • Alpha-L-iduronate sulfate sulfatase antibody
    • AW214631 antibody
    • Ids antibody
    • IDS_HUMAN antibody
    • Iduronate 2 sulfatase 14 kDa chain antibody
    • Iduronate 2 sulfatase 42 kDa chain antibody
    • Iduronate 2 sulfatase antibody
    • Iduronate 2-sulfatase 14 kDa chain antibody
    • Iduronate sulfatase antibody
    • Idursulfase antibody
    • MPS2 antibody
    • RP23-29M4.1 antibody
    • SIDS antibody
    see all

Anti-Iduronate 2 sulfatase antibody 画像

  • Anti-Iduronate 2 sulfatase antibody (ab85701) at 0.1 µg/ml + Human Liver lysate at 35 µg

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab85701 (3.8µg/ml) staining of paraffin embedded Human Placenta shows lysosomal staining of trophoblasts. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-Iduronate 2 sulfatase antibody (ab85701) 使用論文

ab85701 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85701.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"