製品の概要

  • 製品名Anti-IDUA antibody
    IDUA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IDUA
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 244-274 of Human IDUA (NP_000194.2).

  • ポジティブ・コントロール
    • HeLa cell lysate. IF/ICC: HepG2 cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 73 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 関連性IDUA is an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H) also known as Hurler syndrome, mucopolysaccharidosis type 1H/S (MPS1H/S) also known as Hurler-Scheie syndrome and mucopolysaccharidosis type 1S (MPS1S) also known as Scheie syndrome. MPS1S is a mild form whilst MPS1H is a severe form of this rare lysosomal storage disease characterized by progressive physical deterioration with urinary excretion of dermatan sulfate and heparan sulfate.
  • 細胞内局在Lysosome
  • 参照データベース
  • 別名
    • Alpha L iduronidase antibody
    • IDA antibody
    • Iduronidase alpha L antibody
    • MPS1 antibody

Anti-IDUA antibody 画像

  • ab103949 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103949 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-IDUA antibody (ab103949) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 73 kDa

Anti-IDUA antibody (ab103949) 使用論文

ab103949 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab103949.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"