製品の概要

  • 製品名Anti-IDH2 antibody
    IDH2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IDH2
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans
  • 免疫原

    Synthetic peptide, corresponding to a region within internal sequence amino acids 143-192 (GGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTF K) of Human IDH2 (NP_002159)

  • ポジティブ・コントロール
    • Human fetal muscle lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab84726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human IDH2 peptide (ab111673). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-IDH2 antibody 画像

  • Anti-IDH2 antibody (ab84726) at 1 µg/ml + Human fetal muscle lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • ICC/IF image of ab84726 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84726, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab84726 staining in Hu Colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-IDH2 antibody (ab84726) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish skeletal muscle homogenate (20ug)
    Lane 3 : JURKAT (Human T cell lymphoblast-like cell line) Whole Cell Lysate (20ug)

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes
  • Anti-IDH2 antibody (ab84726) at 1/1000 dilution + rat proximal kidney tubules purified from the cortex at 1 µg/ml

    Predicted band size : 51 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

Anti-IDH2 antibody (ab84726) 使用論文

This product has been referenced in:
  • Saha SK  et al. Mutant IDH inhibits HNF-4a to block hepatocyte differentiation and promote biliary cancer. Nature 513:110-4 (2014). Read more (PubMed: 25043045) »
  • Vohwinkel CU  et al. Elevated CO2 Levels Cause Mitochondrial Dysfunction and Impair Cell Proliferation. J Biol Chem 286:37067-76 (2011). WB ; Human . Read more (PubMed: 21903582) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Retina, heart, liver)
Gel Running Conditions Reduced Denaturing (10% resolving gel)
Loading amount 25 µg
Treatment Homogenized and sonicated in MPER
Specification Retina, heart, liver
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Jul 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (Retina, heart)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 25 µg
Treatment M-PER for 30 minutes
Specification Retina, heart
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"