Anti-IDH2 抗体 (ab109588)
Key features and details
- Goat polyclonal to IDH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-IDH2 antibody
IDH2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IDH2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Rabbit, Horse, Chicken, Cow, Dog, Macaque monkey -
免疫原
Synthetic peptide:
CIHGLSNVKLNE
, corresponding to internal sequence amino acids 418-429 of Human IDH2 according to NP_002159.2 -
ポジティブ・コントロール
- Human, Mouse or Rat Heart lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109588の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
関連疾患
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
配列類似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻訳後修飾
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 327669 Cow
- Entrez Gene: 3418 Human
- Entrez Gene: 269951 Mouse
- Entrez Gene: 397603 Pig
- Entrez Gene: 361596 Rat
- Omim: 147650 Human
- SwissProt: Q04467 Cow
- SwissProt: P48735 Human
see all -
別名
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
see all
画像
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All lanes : Anti-IDH2 antibody (ab109588) at 0.1 µg/ml
Lane 1 : Mouse heart tissue lysates
Lane 2 : Rat heart tissue lysates
Lane 3 : Pig heart tissue lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDaPrimary incubation was 1 hour.
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Anti-IDH2 antibody (ab109588) at 0.1 µg/ml + Human heart lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab109588 は 1 報の論文で使用されています。
- Feng L et al. Icariside II alleviates oxygen-glucose deprivation and reoxygenation-induced PC12 cell oxidative injury by activating Nrf2/SIRT3 signaling pathway. Biomed Pharmacother 103:9-17 (2018). PubMed: 29635133