製品の概要

  • 製品名Anti-ID4 antibody
    ID4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ID4
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide from within a region of internal sequence amino acids 71-120 (YSRLRRLVPT IPPNKKVSKV EILQHVIDYI LDLQLALETH PALLRQPPPP) of Human ID4 (NP_001537)

  • ポジティブ・コントロール
    • 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-ID4 antibody 画像

  • Anti-ID4 antibody (ab86113) at 1 µg/ml (5% skim milk/PBS buffer) + 293T cell lysate at 10 µg with gel concentration: 10-20%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa
    Observed band size : 18 kDa

Anti-ID4 antibody (ab86113) 使用論文

This product has been referenced in:
  • Tamaki S & Tokumoto Y Overexpression of cyclin dependent kinase inhibitor P27/Kip1 increases oligodendrocyte differentiation from induced pluripotent stem cells. In Vitro Cell Dev Biol Anim 50:778-85 (2014). WB ; Mouse . Read more (PubMed: 24764126) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, postnatal day 6
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Sep 15 2016

Thank you for confirming these details and for your cooperation.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the a...

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Thank you very much for your reply.

I will be happy to send you a free of charge replacement vial. Unfortunately we do not have another lot of the ab86113 in stock. Would you like to receive instead a free unit of eitherthe ab49261 or the ab1...

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Thank you very much for your email.

I am sorry that you experienced problems with this antibody.

I double checked with the laboratory about the expression data of ID4 in 293T cells., We can confirm this antibody did work when using ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"