Anti-ID3 抗体 (ab86017)
Key features and details
- Rabbit polyclonal to ID3
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
製品の概要
-
製品名
Anti-ID3 antibody
ID3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ID3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
3 synthetic peptides (Human) conjugated to KLH
-
ポジティブ・コントロール
- Recombinant protein produced in an induced culture of E.coli.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading...
-
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86017の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 13 kDa.
|
特記事項 |
---|
WB
1/1000. Predicted molecular weight: 13 kDa. |
ターゲット情報
-
機能
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. -
組織特異性
Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
-
別名
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
see all
画像
-
All lanes : Anti-ID3 antibody (ab86017) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
All lanes : Anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 124 kDa, 209 kDa, 32 kDa, 45 kDa, 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 10%Predicted molecular weight of recombinant ID3:27kDa (13kDa + another 14kDa for the tag).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab86017 は 1 報の論文で使用されています。
- Chen FF et al. miR-212-5p exerts tumor promoter function by regulating the Id3/PI3K/Akt axis in lung adenocarcinoma cells. J Cell Physiol 235:7273-7282 (2020). PubMed: 32039486