製品の概要

  • 製品名
    Anti-ID3 antibody
    ID3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ID3
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    3 synthetic peptides (Human) conjugated to KLH

  • ポジティブ･コントロール
    • Recombinant protein produced in an induced culture of E.coli.

法規制情報

医薬用外毒物

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 13 kDa.

ターゲット情報

  • 機能
    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • 組織特異性
    Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 1R21 antibody
      • bHLHb25 antibody
      • Class B basic helix-loop-helix protein 25 antibody
      • DNA binding protein inhibitor ID 3 antibody
      • DNA-binding protein inhibitor ID-3 antibody
      • HEIR 1 antibody
      • HEIR1 antibody
      • Helix loop helix protein HEIR 1 antibody
      • Helix loop helix protein HEIR1 antibody
      • Helix-loop-helix protein HEIR-1 antibody
      • HLH642 antibody
      • ID 3 antibody
      • ID like protein inhibitor HLH 1R21 antibody
      • ID like protein inhibitor HLH 462 antibody
      • ID-like protein inhibitor HLH 1R21 antibody
      • ID3 antibody
      • ID3_HUMAN antibody
      • Inhibitor of DNA binding 3 antibody
      • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
      see all

    画像

    • Western blot - ID3 antibody (ab86017)
      Western blot - ID3 antibody (ab86017)
      All lanes : Anti-ID3 antibody (ab86017) at 1/1000 dilution

      Lane 1 : Uninduced (negative control) culture of E.coli
      Lane 2 : Induced culture of E.coli

      Secondary
      All lanes : Anti-Rabbit IgG HRP conjugated at 1/1000 dilution

      Predicted band size: 13 kDa
      Observed band size: 36 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 124 kDa, 209 kDa, 32 kDa, 45 kDa, 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.



      Gel concentration: 10%

      Predicted molecular weight of recombinant ID3:27kDa (13kDa + another 14kDa for the tag).

    プロトコール

    参考文献

    ab86017 has not yet been referenced specifically in any publications.

    ab86017 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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