Anti-ID3 抗体 (ab41834)
Key features and details
- Rabbit polyclonal to ID3
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ID3 antibody
ID3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ID3 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
-
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human ID3 (C terminal).
-
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41834の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 1 µg/ml.
|
|
IHC-P | (1) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
特記事項 |
---|
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
機能
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. -
組織特異性
Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 3399 Human
- Entrez Gene: 15903 Mouse
- Omim: 600277 Human
- SwissProt: Q02535 Human
- SwissProt: P41133 Mouse
- Unigene: 76884 Human
- Unigene: 110 Mouse
-
別名
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
see all
画像
-
Ab41834 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.
-
ICC/IF image of ab41834 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab41834 は 7 報の論文で使用されています。
- Hu L et al. Bone Morphogenetic Protein 4 Alleviates DSS-Induced Ulcerative Colitis Through Activating Intestinal Stem Cell by Target ID3. Front Cell Dev Biol 9:700864 (2021). PubMed: 34692671
- Wang X et al. Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level. Cell Res 30:1109-1126 (2020). PubMed: 32690901
- Xu C et al. The microRNA-10a/ID3/RUNX2 axis modulates the development of Ossification of Posterior Longitudinal Ligament. Sci Rep 8:9225 (2018). PubMed: 29907859
- Zywitza V et al. Single-Cell Transcriptomics Characterizes Cell Types in the Subventricular Zone and Uncovers Molecular Defects Impairing Adult Neurogenesis. Cell Rep 25:2457-2469.e8 (2018). PubMed: 30485812
- Forni PE et al. The indirect role of fibroblast growth factor-8 in defining neurogenic niches of the olfactory/GnRH systems. J Neurosci 33:19620-34 (2013). Mouse . PubMed: 24336726
- Snyder AD et al. Expression pattern of id proteins in medulloblastoma. Pathol Oncol Res 19:437-46 (2013). PubMed: 23397264
- Gajera CR et al. LRP2 in ependymal cells regulates BMP signaling in the adult neurogenic niche. J Cell Sci 123:1922-30 (2010). IHC-Fr ; Mouse . PubMed: 20460439