製品の概要

  • 製品名Anti-Id1 antibody
    Id1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Id1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus Id1.

    (Peptide available as ab86654.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: HepG2 Whole Cell, Mouse Pancreas Tissue, Mouse Liver Tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 16 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 発生段階Expression correlates with proliferation in some types of cells.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHb24 antibody
    • Class B basic helix-loop-helix protein 24 antibody
    • dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody
    • DNA binding protein inhibitor ID 1 antibody
    • DNA binding protein inhibitor ID1 antibody
    • DNA-binding protein inhibitor ID-1 antibody
    • Dominant negative helix loop helix protein antibody
    • ID 1 antibody
    • ID antibody
    • ID1 antibody
    • ID1_HUMAN antibody
    • Inhibitor of Differentiation 1 antibody
    • Inhibitor of DNA binding 1 antibody
    • inhibitor of DNA binding 1, dominant negative helix-loop-helix protein antibody
    see all

Anti-Id1 antibody 画像

  • ICC/IF image of ab52998 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52998 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Id1 antibody (ab52998) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    The 22 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Id1.

Anti-Id1 antibody (ab52998) 使用論文

ab52998 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"