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RabMAb

Anti-ICAM3 抗体 [EPR3994] (ab109405)

製品の概要

  • 製品名
    Anti-ICAM3 antibody [EPR3994]
    ICAM3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3994] to ICAM3
  • アプリケーション
    適用あり: ICC, WB, IP, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in the extracellular domain of Human ICAM3.

  • ポジティブ・コントロール
    • U937, Jurkat, HuT-78, Ramos, and THP-1 cell lysates; Human spleen, colon and tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾
    Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
    N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD50 antibody
    • CDW 50 antibody
    • CDw50 antibody
    • ICAM 3 antibody
    • ICAM-3 antibody
    • ICAM-R antibody
    • ICAM3 antibody
    • ICAM3_HUMAN antibody
    • ICAMR antibody
    • Intercellular adhesion molecule 3 antibody
    see all

画像

  • All lanes : Anti-ICAM3 antibody [EPR3994] (ab109405) at 1/1000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HuT-78 cell lysate
    Lane 4 : Ramos cell lysate
    Lane 5 : THP-1 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human spleen tissue using ab109405 at 1/500 dilution.
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human colon tissue using ab109405 at 1/500 dilution.
  • Immunohistochemical analysis of ICAM3 in paraffin-embedded Human tonsil tissue using ab109405 at 1/500dilution.

参考文献

ab109405 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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