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Our Abpromise guarantee covers the use of ab25246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
ab25246 staining ICAM2 in mouse olfactory epithelium tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 1% paraformaldehyde + lysine + periodate (1% PLP), permeablized and blocked with 10% donkey serum + 5% nonfat dry milk + 4% BSA + 1% triton X-100 and antigen retrieval used 5% H2O2 in MeOH for 5 minutes room temperature. The sample was incubated with primary antibody (1/200) at 27°C for 1 hour. An Alexa Fluor® 488-conjugated donkey anti-rat polyclonal (1/150) was used as the secondary antibody.
ab25246 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"