製品の概要

  • 製品名Anti-ICAM2 antibody [3C4]
    ICAM2 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [3C4] to ICAM2
  • アプリケーション適用あり: IHC-FoFr, Flow Cyt, IHC-Fr, Blocking, IPmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    COS cells transfected with Mouse ICAM2 cDNA.

  • 特記事項


    The 3C4 antibody blocks the interaction between ICAM2 and LFA1.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 一次抗体 備考The 3C4 antibody blocks the interaction between ICAM2 and LFA1.
  • ポリ/モノモノクローナル
  • クローン名3C4
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/200.
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
  • 細胞内局在Membrane; Single-pass type I membrane protein
  • 参照データベース
  • 別名
    • CD102 antibody
    • CD102 antigen antibody
    • ICAM 2 antibody
    • Intercellular adhesion molecule 2 antibody
    • Ly60 antibody
    see all

Anti-ICAM2 antibody [3C4] 画像

  • ab25246 staining ICAM2 in mouse olfactory epithelium tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 1% paraformaldehyde + lysine + periodate (1% PLP), permeablized and blocked with 10% donkey serum + 5% nonfat dry milk + 4% BSA + 1% triton X-100 and antigen retrieval used 5% H2O2 in MeOH for 5 minutes room temperature. The sample was incubated with primary antibody (1/200) at 27°C for 1 hour. An Alexa Fluor® 488-conjugated donkey anti-rat polyclonal (1/150) was used as the secondary antibody.

    See Abreview

Anti-ICAM2 antibody [3C4] (ab25246) 使用論文

ab25246 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step Other
Sample Mouse Tissue sections (Olfactory epithelium)
Specification Olfactory epithelium
Permeabilization Yes - .1% TritonX-100 in block
Fixative 1% Paraformaldehyde+lysine+periodate (1%PLP)
Username

Dr. Schwob Lab

Verified customer

投稿 Jun 11 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"