製品の概要

  • 製品名Anti-ICAM1 antibody [YN1/1.7.4]
    ICAM1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [YN1/1.7.4] to ICAM1
  • アプリケーション適用あり: WB, IP, IHC-Fr, Functional Studies, ICC, Flow Cyt, Blocking, IHC-P, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Mouse NS-1 cells.

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Mouse lung.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Application note:acetone fixation
Functional Studies Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Blocking Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-FoFr 1/200.

ターゲット情報

  • 機能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • 配列類似性Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Anti-ICAM1 antibody [YN1/1.7.4] 画像

  • IHC image of ICAM1 staining in Mouse normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution, (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab119871, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab119871 staining ICAM1 in mouse olfactory epithelium tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with 1% paraformaldehyde + lysine + periodate (1% PLP) and permeablized and blocked with 10% donkey serum + 5% nonfat dry milk + 4% BSA + 1% triton X-100. The sample was incubated with primary antibody (1/200) for 1 hour at 27°C. An Alexa Fluor® 488-conjugated donkey anti-rat polyclonal (1/150) was used as the secondary antibody.

    See Abreview

Anti-ICAM1 antibody [YN1/1.7.4] (ab119871) 使用論文

This product has been referenced in:
  • Sun H  et al. Attenuation of atherosclerotic lesions in diabetic apolipoprotein E-deficient mice using gene silencing of macrophage migration inhibitory factor. J Cell Mol Med 19:836-49 (2015). IHC-P ; Mouse . Read more (PubMed: 25661015) »
  • Weber EW  et al. TRPC6 is the endothelial calcium channel that regulates leukocyte transendothelial migration during the inflammatory response. J Exp Med 212:1883-99 (2015). Read more (PubMed: 26392222) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Mouse Tissue sections (Olfactory epithelium)
Specification Olfactory epithelium
Permeabilization Yes - .1% TritonX-100 in block
Fixative 1% Paraformaldehyde+lysine+periodate (1%PLP)
Username

Dr. Schwob Lab

Verified customer

投稿 Jun 10 2014

Thank you for contacting us.

The predicted size, based on the amino acid sequence before any post-translation modifications, is 58 kDa. I do not have the western blot data for this particular antibody, but 58 kDa is the size of the proteins ...

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Thanks you for your email.
In my previous email there is a correction about the expiry date. The expiry date should be read as 25-08-2012.
I have asked lab to release the immunogen information of these antibodies; I will be able to send the a...

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