Anti-ICAM1 抗体 [YN1/1.7.4] (ab119871)
Key features and details
- Rat monoclonal [YN1/1.7.4] to ICAM1
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG2b
製品の概要
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製品名
Anti-ICAM1 antibody [YN1/1.7.4]
ICAM1 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [YN1/1.7.4] to ICAM1 -
由来種
Rat -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Tissue, cells or virus corresponding to Mouse ICAM1. Mouse NS-1 cells
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ポジティブ・コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Mouse lung.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purity is > 95% (y SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
YN1/1.7.4 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt
Use a concentration of 1 µg/ml. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
配列類似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 15894 Mouse
- Entrez Gene: 25464 Rat
- SwissProt: P13597 Mouse
- SwissProt: Q00238 Rat
- Unigene: 435508 Mouse
- Unigene: 12 Rat
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別名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse atheroslerotic lesions of aortic root tissue sections labeling ICAM1 with ab119871 at 1/200 dilution. The aortic root was rapidly fixed and harvested at 4°C in phosphate-buffered 4% paraformaldehyde, pH 7.0–7.4, then embedded in paraffin wax sectioned by semi-automated rotary microtome.
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IHC image of ICAM1 staining in Mouse normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution, (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab119871, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Flow Cytometry analysis of mouse thymocytes labeling ICAM1 with Anti-ICAM1 antibody [YN1/1.7.4] (ab119871).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (56)
ab119871 は 56 報の論文で使用されています。
- Ding P et al. BMPER alleviates ischemic brain injury by protecting neurons and inhibiting neuroinflammation via Smad3-Akt-Nrf2 pathway. CNS Neurosci Ther 28:593-607 (2022). PubMed: 34904361
- Choe YG et al. Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain. Front Cell Neurosci 16:848764 (2022). PubMed: 35360491
- Kataru RP et al. Structural and Functional Changes in Aged Skin Lymphatic Vessels. Front Aging 3:864860 (2022). PubMed: 35821848
- Zhou Y et al. Adiponectin Prevents Restenosis Through Inhibiting Cell Proliferation in a Rat Vein Graft Model. Arq Bras Cardiol 117:1179-1188 (2021). PubMed: 35613174
- McDowell SAC et al. Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration. Nat Cancer 2:545-562 (2021). PubMed: 35122017