製品の概要

  • 製品名Anti-ICAM1 antibody [EPR4776]
    ICAM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4776] to ICAM1
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cytmore details
    適用なし: ICC
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to a region near the C-terminus of Human ICAM1.

  • ポジティブ・コントロール
    • Lysates of the following cells: Raji, Ramos, A549 treated with TNF-alpha, A549, and HUVEC treated with TNF-alpha; Human kidney tissue and Human tonsil tissue.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC.
  • ターゲット情報

    • 機能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • 配列類似性Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • 翻訳後修飾Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    Anti-ICAM1 antibody [EPR4776] 画像

    • Overlay histogram showing Ramos cells stained with ab109361 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109361, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-ICAM1 antibody [EPR4776] (ab109361) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Ramos cell lysate
      Lane 3 : A549 cells treated with TNF-alpha, lysate
      Lane 4 : A549 cell lysate
      Lane 5 : HUVEC cells treated with TNF-alpha, lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 58 kDa
      Observed band size : 89 kDa (why is the actual band size different from the predicted?)
    • ab109361, at 1/100, staining ICAM1 in Human kidney tissue by immunohistochemistry.
    • ab1091361, at 1/100, staining ICAM1 in Human tonsil tissue by immunohistochemistry.

    Anti-ICAM1 antibody [EPR4776] (ab109361) 使用論文

    This product has been referenced in:
    • Effenberger T  et al. Senescence-associated release of transmembrane proteins involves proteolytic processing by ADAM17 and microvesicle shedding. FASEB J 28:4847-56 (2014). Read more (PubMed: 25077560) »
    • Huang WM  et al. Antioxidant and anti-inflammatory effects of Astragalus polysaccharide on EA.hy926 cells. Exp Ther Med 6:199-203 (2013). WB ; Human . Read more (PubMed: 23935746) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us. Your credit note ID is I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a f...

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    Thank you for the response.

    Any monoclonal antibody is produced by a single immune cell, which has been cloned. These will only recognize a single epitope of the target protein. This epitope typically is 5-10 amino acids in length. Therefore...

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    While many of our ICAM1 antibodies have been tested in Western blot on human and are guaranteed to work under the terms of our Abpromise, in this instance I would recommend one of the following products. ab96485:http://www.abcam.com/ICAM1-antibody-6...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"