製品の概要

  • 製品名Anti-ICAM1 antibody [EP1442Y]
    ICAM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1442Y] to ICAM1
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ICAM1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • Raji cell membrane lysate Human tonsil tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-ICAM1 antibody (HRP) [EP1442Y] (ab195528)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 89 kDa (predicted molecular weight: 58 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
    • 配列類似性Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • 翻訳後修飾Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Antigen identified by monoclonal antibody BB2 antibody
      • BB 2 antibody
      • BB2 antibody
      • CD 54 antibody
      • CD_antigen=CD54 antibody
      • CD54 antibody
      • Cell surface glycoprotein P3.58 antibody
      • Human rhinovirus receptor antibody
      • ICAM 1 antibody
      • ICAM-1 antibody
      • ICAM1 antibody
      • ICAM1_HUMAN antibody
      • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
      • Intercellular adhesion molecule 1 antibody
      • Major group rhinovirus receptor antibody
      • MALA 2 antibody
      • MALA2 antibody
      • MyD 10 antibody
      • MyD10 antibody
      • P3.58 antibody
      • Surface antigen of activated B cells, BB2 antibody
      see all

    Anti-ICAM1 antibody [EP1442Y] 画像

    • ab53013, at a 1/50 dilution, staining Human ICAM1 in Tonsil using Immunohistochemistry, Paraffin embedded tissue.
    • All lanes : Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/1000 dilution

      Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 4 : THP1 (Human acute monocytic leukemia cell line) + LPS Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 58 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)


      Exposure time : 20 minutes

      s produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab53013 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Overlay histogram showing Raji cells stained with ab53013 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53013, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Anti-ICAM1 antibody [EP1442Y] (ab53013) at 1/2000 dilution + Raji cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size : 58 kDa
      Observed band size : 89 kDa (why is the actual band size different from the predicted?)

    Anti-ICAM1 antibody [EP1442Y] (ab53013) 使用論文

    This product has been referenced in:
    • Shi H  et al. Long non-coding RNA expression profile in minor salivary gland of primary Sjögren's syndrome. Arthritis Res Ther 18:109 (2016). IHC ; Human . Read more (PubMed: 27188286) »
    • Sumbria RK  et al. A murine model of inflammation-induced cerebral microbleeds. J Neuroinflammation 13:218 (2016). IHC ; Mouse . Read more (PubMed: 27577728) »

    See all 12 Publications for this product

    Product Wall

    Thank you for contacting us.

    The concentration for lot GR27346-6 is XXXX mg/ml.

    The conc. for lot GR10519-3 is XXXX mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more a...

    Read More

    Thank you for calling Abcam earlier today.

    I am sorry that you were having issue with ab53013 in IHC. As we discussed on the telephone, I am sending you a vial of ab109361 as a free of charge replacement. Your new order number for tracking pur...

    Read More

    Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

    Weare pleased to arrange a replacement product free of charge. I have added now one free unit of ab53013,xx We are currently awai...

    Read More

    Thank you very muchfor taking time to complete our questionnaire and the additional information. Even though we have not yet tested this antibody in frozen sections, I would expect the new batch to work as the previous one as this is a monoclonal antib...

    Read More

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We have not received any product complaints of this antibody during the past three years and have also no other indication that there...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"