製品の概要

  • 製品名Anti-ICAM1 antibody [1H4]
    ICAM1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1H4] to ICAM1
  • アプリケーション適用あり: Flow Cyt, WB, IHC-P, IHC-Fr, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Raji cells and spleen cells fused with NS1 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 90 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Human tonsil tissue sections could be used as positive control. 5 minutes heating in microwave in Na Citrate buffer pH 6.0 is recommended for antigen retrieval.
IHC-Fr Use a concentration of 5 - 10 µg/ml.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
  • 配列類似性Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻訳後修飾Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen identified by monoclonal antibody BB2 antibody
    • BB 2 antibody
    • BB2 antibody
    • CD 54 antibody
    • CD_antigen=CD54 antibody
    • CD54 antibody
    • Cell surface glycoprotein P3.58 antibody
    • Human rhinovirus receptor antibody
    • ICAM 1 antibody
    • ICAM-1 antibody
    • ICAM1 antibody
    • ICAM1_HUMAN antibody
    • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody
    • Intercellular adhesion molecule 1 antibody
    • Major group rhinovirus receptor antibody
    • MALA 2 antibody
    • MALA2 antibody
    • MyD 10 antibody
    • MyD10 antibody
    • P3.58 antibody
    • Surface antigen of activated B cells, BB2 antibody
    see all

Anti-ICAM1 antibody [1H4] 画像

  • Overlay histogram showing Ramos cells stained with ab67217 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67217, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Ramos cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-ICAM1 antibody [1H4] (ab67217) 使用論文

This product has been referenced in:
  • Su B  et al. Dendritic cell-lymphocyte cross talk downregulates host restriction factor SAMHD1 and stimulates HIV-1 replication in dendritic cells. J Virol 88:5109-21 (2014). Human . Read more (PubMed: 24574390) »
  • Holl V  et al. Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes. J Virol 84:4172-82 (2010). Flow Cyt ; Human . Read more (PubMed: 20147388) »

See all 2 Publications for this product

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