製品の概要

  • 製品名Anti-IBRDC2 antibody [4F1]
    IBRDC2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4F1] to IBRDC2
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-101 of Human IBRDC2 with proprietary tag (NP_877434).

  • ポジティブ・コントロール
    • Human placenta, liver and kidney tissues; HeLa clls; IMR32 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se.
  • 組織特異性Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the RBR family. RNF144 subfamily.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • ドメインThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme (By similarity). The transmembrane domain is essential for translocation to the mitochondria upon induction of apoptosis.
  • 翻訳後修飾Auto-ubiquitinated.
  • 細胞内局在Mitochondrion membrane. Cytoplasm. Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA528A10.3 antibody
    • E3 ubiquitin protein ligase RNF144B antibody
    • E3 ubiquitin-protein ligase RNF144B antibody
    • IBR domain containing 2 antibody
    • IBR domain containing protein 2 antibody
    • IBR domain-containing protein 2 antibody
    • IBRDC 2 antibody
    • IBRDC2 antibody
    • KIAA0161 antibody
    • MGC71786 antibody
    • OTTHUMP00000016079 antibody
    • OTTHUMP00000016080 antibody
    • OTTHUMP00000039360 antibody
    • p53 inducible RING finger protein antibody
    • p53-inducible RING finger protein antibody
    • p53RFP antibody
    • PIR2 antibody
    • R144B_HUMAN antibody
    • Ring finger 144B antibody
    • RING finger protein 144B antibody
    • RNF 144B antibody
    • RNF144B antibody
    see all

Anti-IBRDC2 antibody [4F1] 画像

  • Anti-IBRDC2 antibody [4F1] (ab110458) + IMR32 cell lysate

    Predicted band size : 34 kDa
  • Immunofluorescence of IBRDC2 expression in HeLa cells using ab110458 at 10 µg/ml.
  • Analysis of IBRDC2 expression in formalin-fixed, paraffin-embedded Human kidney tissue using ab110458 at 5µg/ml.
  • Analysis of IBRDC2 expression in formalin-fixed, paraffin-embedded Human liver tissue using ab110458 at 5µg/ml.
  • Analysis of IBRDC2 expression in formalin-fixed, paraffin-embedded Human placenta tissue using ab110458 at 5µg/ml.

Anti-IBRDC2 antibody [4F1] (ab110458) 使用論文

ab110458 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"