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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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HEK293 cells plated for 48 hours and then transfected with rat Slo27 for 24 hours. Currents from whole-cell recordings, with 1 µM Ca2+ in the electrode solution. Cells voltage-clamped at -80 mV, with currents activated by 500 ms depolarization to +20 mV. Concentration-inhibition relationship produces an IC50 of 412 pM.
(Number of experimental determinations shown in brackets).
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