製品の概要

  • 製品名Anti-Iba1 antibody
    Iba1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Iba1
  • 特異性This antibody, ab5076, is expected to recognise the isoforms represented by NP_001614 and NP_116573 but not NP_004838 Please note: Although we have some very good Abreviews on mouse, some customers were receiving inconsistent results on mouse samples. We have therefore moved mouse to the predicted species and can no longer guarantee it. In addition, we recommend blocking in milk. Blocking with BSA gives high background. IHC-Fr. Some customers have used this product in IHC-Fr however we have had consistent complaints in this application over the last year and can no longer guarantee performance in this application. If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846. This has the same immunogen as ab5076.
  • アプリケーション適用あり: IHC-P, WB, IHC-FrFl, ICC, ICC/IFmore details
    適用なし: IHC-FoFr
  • 種交差性
    交差種: Rat, Rabbit, Guinea pig, Cow, Human, Pig, Marmoset (common)
    交差が予測される動物種: Mouse, Macaque Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Iba1 aa 135-147 (C terminal). Accession Number(s): NP_116573.1; NP_001614.3
    Sequence:

    C-TGPPAKKAISELP


    (Peptide available as ab23067)

  • 特記事項

    Please note: Although we have some very good Abreviews on mouse, some customers were receiving inconsistent results on mouse samples. We have therefore moved mouse to the predicted species and can no longer guarantee it.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab5076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1.5 - 4.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).Can be blocked with Human Iba1 peptide (ab23067).

We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk. Blocking with BSA gives high background.

IHC-FrFl Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • 追加情報Is unsuitable for IHC-FoFr.
  • ターゲット情報

    • 機能Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
    • 組織特異性Detected in T-lymphocytes and peripheral blood mononuclear cells.
    • 配列類似性Contains 2 EF-hand domains.
    • 翻訳後修飾Phosphorylated on serine residues.
    • 細胞内局在Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AIF 1 antibody
      • AIF-1 antibody
      • Aif1 antibody
      • AIF1 protein antibody
      • AIF1_HUMAN antibody
      • Allograft inflammatory factor 1 antibody
      • Allograft inflammatory factor 1 splice variant G antibody
      • allograft inflammatory factor-1 splice variant Hara-1 antibody
      • balloon angioplasty responsive transcription antibody
      • BART 1 antibody
      • G1 antibody
      • G1 putative splice variant of allograft inflamatory factor 1 antibody
      • IBA 1 antibody
      • IBA1 antibody
      • interferon gamma responsive transcript antibody
      • Interferon responsive transcript 1 antibody
      • interferon responsive transcript factor 1 antibody
      • Ionized calcium binding adapter molecule 1 antibody
      • Ionized calcium-binding adapter molecule 1 antibody
      • ionized calcium-binding adapter molecule antibody
      • IRT 1 antibody
      • IRT1 antibody
      • Microglia response factor antibody
      • MRF1 antibody
      • Protein g1 antibody
      see all

    Anti-Iba1 antibody 画像

    • IHC-P image of Iba1 staining on rat brain sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)

      See Abreview

    • All lanes : Anti-Iba1 antibody (ab5076) at 1 µg/ml

      Lane 1 : Human Iba1 full length recombinant protein at 0.1 µg
      Lane 2 : HEK293 whole cell lysate at 30 µg
      Lane 3 : A431 whole cell lysate at 30 µg
      Lane 4 : NIH3T3 whole cell lysate at 30 µg
      Lane 5 : Human spleen tissue lysate at 30 µg
      Lane 6 : Mouse spleen tissue lysate at 30 µg
      Lane 7 : Rat spleen tissue lysate at 30 µg
      Lane 8 : U937 whole cell lysate at 30 µg
      Lane 9 : HL60 whole cell lysate at 30 µg
      Lane 10 : THP1 whole cell lysate at 30 µg
      Lane 11 : THP1 whole cell lysate, PMA treated at 30 µg
      Lane 12 : Raw 264.7 whole cell lysate at 30 µg
      Lane 13 : C6 whole cell lysate at 30 µg
      Lane 14 : NR8383 whole cell lysate at 30 µg
      Lane 15 : Mouse spleen tissue lysate at 30 µg
      Lane 16 : THP1 whole cell lysate at 30 µg

      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 17 kDa

      Lanes 1-14: Blocked in 3% milk for 1 hour (RT). Lanes 15-16: Blocked in 5% BSA for 1 hour (RT).

      Lane 1: exposure 1 minute. Lanes 2-16: exposure 4 minutes. Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA. 

      This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab5076 (anti-Iba1 antibody; 1 ug/mL) for 18 hours at 4°C. 

    • ab5076 staining Iba1 in the Macrophage cell line Mono-Mac 6 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Rabbit anti-goat IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • IHC-P image of Iba1 staining on cat kidney sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)

      See Abreview

    • Anti-Iba1 antibody (ab5076) at 0.3 µg/ml + Rat brain lysate at 35 µg

      Predicted band size : 17 kDa
      Observed band size : 17 kDa

      Primary incubation was 1 hour. Detected by chemiluminescence.

    • ab5076 staining Iba1 in rat glioblastoma cell line C6 by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0,1% Triton X 100 in PBS, blocked with 0.5% BSA for 30 minutes at room temperature and then incubated with ab5076 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a Cy3 conjugated rabbit anti-goat polyclonal used at a 1/120 dilution. Nuclei are counterstained with DAPI.

      See Abreview

    • IHC-P image of Iba1 staining on cow kidney sections using ab5076 (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:2000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti sheep/goat IgG conjugated to biotin (1:200)

      See Abreview

    • IHC-P image of Iba1 staining on marmoset bladder sections using ab5076 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab5076 was diluted 1:1000 and incubted with the sections for 2 hours at 21°C. The secondary antibody used was Rabbit polyclonal to anti goat IgG conjugated to biotin (1:200)

      See Abreview

    • AIF1 expression in an inflammatory foci in a skin biopsy. Paraffin embedded sections were deparaffinized and boiled in 10mM citrate buffer for 30 min in a microwave to expose the AIF1 antigen, and then blocked with 5% donkey serum for 20 min. Slides were incubated for 40 min with goat-anti-AIF1 antibody (1:100) and rinsed in three changes of PBS for 1 min each.  A secondary antibody (donkey-anti-goat-FITC conjugated IgG; 1:50) was then applied to the slide for 40 min. Sections were washed again to remove the unbound antibody. The slides were counterstained with DaPI and viewed with a Nikon epi-fluorescent microscope. AIF1 positive cells appear green and the nuclei are stained blue. Numerous cells expressing the AIF1 protein are located around a vessel. (400X). Picture from Rreview by Carol Artlett submitted 30 July 2004.
    • ab5076 staining Iba1 in guinea pig brain tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval in sodium citrate and then blocking with 3% serum was performed for 30 minutes at RT. The primary antibody was used at dilution at 1/200 and incubated with sample at 2% blocking serum for 18 hours at 4°C. A Biotin conjugated horse polyclonal to goat IgG was used undiluted as secondary antibody.

      See Abreview

    Anti-Iba1 antibody (ab5076) 使用論文

    This product has been referenced in:
    • Arroba AI  et al. Autophagy resolves early retinal inflammation in Igf1-deficient mice. Dis Model Mech 9:965-74 (2016). WB ; Mouse . Read more (PubMed: 27483352) »
    • Schmidt AF  et al. Intra-amniotic LPS causes acute neuroinflammation in preterm rhesus macaques. J Neuroinflammation 13:238 (2016). IHC-P ; Rhesus monkey . Read more (PubMed: 27596440) »

    See all 89 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain)
    Permeabilization Yes - tritonx100
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative Paraformaldehyde
    Username

    Ms. kyungjoo seong

    Verified customer

    投稿 Nov 02 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (P0 mouse cortex tissue)
    Gel Running Conditions Reduced Denaturing (12% Tris-Glycine)
    Loading amount 50 µg
    Specification P0 mouse cortex tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Ms. Xiaofeng Zhou

    Verified customer

    投稿 Jun 15 2016

    Application Immunohistochemistry free floating
    Sample Mouse Tissue sections (brain - cerebral cortex)
    Specification brain - cerebral cortex
    Username

    Abcam user community

    Verified customer

    投稿 Apr 12 2016

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Spinal cord)
    Antigen retrieval step None
    Permeabilization Yes - 50% ethanol, 15min
    Specification Spinal cord
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Mar 09 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Spinal cord L4,L5 avulsion injury 16 micron thick)
    Permeabilization No
    Specification Spinal cord L4,L5 avulsion injury 16 micron thick
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative Formaldehyde
    Username

    Mr. Tamas Bellak

    Verified customer

    投稿 Dec 07 2015

    The molar concentration of the Tris is 20mM. The formulation of the buffer is Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin.

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
    Permeabilization No
    Specification brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 05 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (Retina)
    Antigen retrieval step Heat mediated
    Permeabilization Yes - Tween
    Specification Retina
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Dra. Marta Garcia Ramirez

    Verified customer

    投稿 Jul 08 2015

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (brain)
    Antigen retrieval step None
    Permeabilization No
    Specification brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Keerthi Thirtamara Rajamani

    Verified customer

    投稿 Jun 15 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Mouse striatal section)
    Permeabilization Yes - Triton X-100 .3%
    Specification Mouse striatal section
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 01 2015

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"