製品の概要

  • 製品名Anti-Iba1 antibody
    Iba1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Iba1
  • 特異性In WB, we recommend blocking in milk. Blocking with BSA gives high background. If looking for a monoclonal anti-Iba1 alternative we can recommend our RabMAb ab178846
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cat, Human
  • 免疫原

    Synthetic peptide from the C terminal amino acids of Human Iba1 (NM_001623.3).

  • ポジティブ・コントロール
    • Iba1 (Human) full length recombinant protein; Rat brain lysate; Human fetal lymphocytes; spleen tissue lysate; THP1, C6, NR8383 whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 17 kDa.

We recommend blocking in 3-5% milk. Blocking with BSA gives high background.

IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
  • 組織特異性Detected in T-lymphocytes and peripheral blood mononuclear cells.
  • 配列類似性Contains 2 EF-hand domains.
  • 翻訳後修飾Phosphorylated on serine residues.
  • 細胞内局在Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIF 1 antibody
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    • BART 1 antibody
    • G1 antibody
    • G1 putative splice variant of allograft inflamatory factor 1 antibody
    • IBA 1 antibody
    • IBA1 antibody
    • interferon gamma responsive transcript antibody
    • Interferon responsive transcript 1 antibody
    • interferon responsive transcript factor 1 antibody
    • Ionized calcium binding adapter molecule 1 antibody
    • Ionized calcium-binding adapter molecule 1 antibody
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    • IRT 1 antibody
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    • Microglia response factor antibody
    • MRF1 antibody
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    see all

Anti-Iba1 antibody 画像

  • IHC image of Iba1 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of Iba1 staining in normal mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of Iba1 staining in normal rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108539, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

  • All lanes : Anti-Iba1 antibody (ab108539) at 1/1000 dilution

    Lane 1 : Human Iba1 recombinant protein at 0.1 µg
    Lane 2 : HEK293 whole cell lysate at 20 µg
    Lane 3 : A431 whole cell lysate at 20 µg
    Lane 4 : NIH3T3 whole cell lysate at 30 µg
    Lane 5 : Human spleen tissue lysate at 20 µg
    Lane 6 : Mouse spleen tissue lysate at 30 µg
    Lane 7 : Rat spleen tissue lysate at 30 µg
    Lane 8 : U937 whole cell lysate at 30 µg
    Lane 9 : MOLT4 whole cell lysate at 20 µg
    Lane 10 : THP1 whole cell lysate at 30 µg
    Lane 11 : THP1 whole cell lysate, PMA treated at 30 µg
    Lane 12 : Raw 264.7 whole cell lysate at 30 µg
    Lane 13 : C6 whole cell lysate at 30 µg
    Lane 14 : NR8383 whole cell lysate at 30 µg
    Lane 15 : NIH3T3 whole cell lysate - BLOCKED IN 5% BSA at 30 µg
    Lane 16 : Human spleen tissue lysate - BLOCKED IN 5% BSA at 20 µg

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa


    Exposure time : 3 minutes

    Lanes 1-14: Blocked in 3% milk for 1 hour (RT). Lanes 15-16: Blocked in 5% BSA for 1 hour (RT).

    Abcam recommends blocking in milk for cleaner blots with reduced background, in comparison to BSA. 

    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with ab108539 (anti-Iba1 antibody; 1/1000) for 18 hours at 4°C. Antibody binding was detected using HRP-labelled anti-Rabbit IgG for 1 hour at room temperature and visualised using ECL development solution ab133406.

  • Anti-Iba1 antibody (ab108539) at 1/500 dilution + Rat brain lysate

    Predicted band size : 17 kDa
  • ab108539, at 1/100 dilution, staining Iba1 in formalin-fixed, paraffin-embedded Human fetal lymphocytes by Immunohistochemistry.

Anti-Iba1 antibody (ab108539) 使用論文

This product has been referenced in:
  • Bryukhovetskiy I  et al. Cancer stem cells and microglia in the processes of glioblastoma multiforme invasive growth. Oncol Lett 12:1721-1728 (2016). Rat . Read more (PubMed: 27602106) »
  • Seelig DM  et al. Lesion Profiling and Subcellular Prion Localization of Cervid Chronic Wasting Disease in Domestic Cats. Vet Pathol N/A:N/A (2014). Cat . Read more (PubMed: 24577721) »

See all 3 Publications for this product

Product Wall

Thank you for contacting Abcam.

So that I can better help you with this, would you be able to provide me with a copy of the image that you are getting and also the protocol that you are using, in particular could you include the following inf...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: MW pH9
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1500µg/mL
Fixative Paraformaldehyde
Username

Gaia Brezzo

Verified customer

投稿 Dec 16 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Liver
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 30 2015

Thank you for contacting us.

I can confirm that antibody ab108539 could be used for detection of Iba1 in microglia cells of mouse brain. This assumption is however based on the expression of this protein in mouse microglia cells; we unfortunat...

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Thank you for taking the time to contact us. I am sorry to hearthe customer hashad difficulty obtaining satisfactory results from these threeantibodies.

I would like to reassure you that in the event that a product is not functioning in the ...

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Here is a quite detailed protocol for performing paraffin embedded immunohistochemical staining, which has the buffer composition for Tris-EDTA pH 9 which I suggested trying: http://www.abcam.com/ps/pdf/protocols/ihc_p.pdf Tris-EDTA buffer: ...

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Thank you for contacting us again. As discussed over the phone, I have arranged for the free of charge replacement with ab5076 to be delivered to you at the following address (taken from your previous order): The new order number is: This s...

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Thank you for your enquiry. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Iba1 antibody tested in the species...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"