製品の概要

  • 製品名Anti-HYPE antibody
    HYPE 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HYPE
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 409-458 (GDVRPFIRFI AKCTETTLDT LLFATTEYSV ALPEAQPNHS GFKETLPVKP) of Human HYPE (NP_009007).

  • ポジティブ・コントロール
    • 293T cells transfected with HYPE cell lysate. Human muscle.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. Able to inactivate Rho GTPases in vitro by adding AMP to RhoA, Rac and Cdc42. It is however unclear whether it inactivates GTPases in vivo and physiological substrates probably remain to be identified.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the fic family.
    Contains 1 fido domain.
    Contains 2 TPR repeats.
  • ドメインThe fido domain mediates the adenylyltransferase activity.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenosine monophosphate-protein transferase FICD antibody
    • AMPylator FICD antibody
    • FIC domain containing antibody
    • FIC domain containing protein antibody
    • FIC domain-containing protein antibody
    • Fic S phase protein cell division homolog antibody
    • ficd antibody
    • FICD_HUMAN antibody
    • HIP-13 antibody
    • HIP13 antibody
    • Huntingtin interacting protein 13 antibody
    • Huntingtin interacting protein E antibody
    • Huntingtin interactor protein E antibody
    • Huntingtin yeast partner E antibody
    • Huntingtin-interacting protein 13 antibody
    • Huntingtin-interacting protein E antibody
    • MGC5623 antibody
    • UNQ3041 antibody
    see all

Anti-HYPE antibody 画像

  • Anti-HYPE antibody (ab104763) at 0.25 µg/ml + transfected 293T cell lysate at 10 µg

    Predicted band size : 52 kDa
    12% gel
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling HYPE with ab104763 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.

Anti-HYPE antibody (ab104763) 使用論文

ab104763 has not yet been referenced specifically in any publications.

Product Wall

Thanks for the additional details.

What speices of HYPE is your construct based on?

Thanks for your enquiry.

Just to confirm, ab104782 has been raised against a synthetic peptide immunogen "corresponding to a region within the C terminal amino acids 351-400". This peptide is a 15-20 amino acid peptide found withina...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"