Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130)

製品の概要

  • 製品名Hydroxymethylated DNA Quantification Kit (Colorimetric)
    hydroxymethylated DNA キット 製品一覧
  • サンプルの種類
    Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 検出感度
    >= 40 pg/well
  • 全工程の試験時間
    3h 45m
  • 製品の概要

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quite recently, a novel modified nucleotide, 5-hydroxymethylcytosine (5-hmC), has been detected to be abundant in mouse brain and embryonic stem cells. It is a hydroxylated and methylated form of cytosine. Although it is still uncertain its specific role in epigenetics, it is believed it plays a role in DNA demethylation, chromatin remodeling and gene expression regulation.

    Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130) is a complete set of optimized buffers and reagents to quantify global DNA hydroxymethylation by specifically measuring levels of 5-hydroxymethylcytosine (5-hmC) without cross-reactivity to methylcytosine and unmethylated cytosine.

    It can be paired to use with Methylated DNA Quantification Kit (Colorimetric) (ab117128), which specifically quantifies 5-mC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 保存方法Please refer to protocols.
  • 内容 48 tests 96 tests
    10X Wash Buffer 1 x 14ml 1 x 28ml
    8-Well Assay Strips (with Frame) 6 units 12 units
    Binding Solution 1 x 5ml 1 x 10ml
    Capture Antibody, 1000 µg/mL 1 x 4µl 1 x 8µl
    Detection Antibody, 400 µg/mL 1 x 8µl 1 x 16µl
    Developer Solution 1 x 5ml 1 x 10ml
    Enhancer Solution 1 x 8µl 1 x 16µl
    Negative Control I, 20 µg/mL 1 x 10µl 1 x 20µl
    Negative Control II, 20 µg/mL 1 x 10µl 1 x 20µl
    Positive Control, 20 µg/mL 1 x 10µl 1 x 20µl
    Stop Solution 1 x 5ml 1 x 10ml
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab117130 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Hydroxymethylated DNA Quantification Kit (Colorimetric) 画像

    • Demonstration of a high abundance of 5-hydroxymethylcytosine in human brain and colon tissues. 200 ng of DNA isolated from different tissues or cells were added into the assay wells and the hydroxymethylated fraction of DNA was measured using the ab117130. OD intensity measured in variety of DNA samples.
    • Demonstration of high specificity of 5-hydroxymethylcytosine detection achieved using ab117130. Synthetic unmethylated DNA (contains only cytosine), methylated DNA (contains only 5-methylcytosine), and hydroxymethylated DNA standard (contains only 5-hydroxymethylcytosine) were added into the assay wells at different concentrations and then measured with ab117130.
    • Demonstration of a high abundance of 5-hydroxymethylcytosine in human brain and colon tissues. 200 ng of DNA isolated from different tissues or cells were added into the assay wells and the hydroxymethylated fraction of DNA was measured using ab117130. Percentage of hydroxymethylated DNA in various tissues and cells.
    • Demonstration of high sensitivity of 5-hydroxymethylcytosine detection achieved using ab117130. Synthetic hydroxymethylated DNA was added into the assay wells at different concentrations and then measured with the ab117130.

    プロトコール

    Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130) 使用論文

    This product has been referenced in:
    • Pérez LM  et al. Altered metabolic and stemness capacity of adipose tissue-derived stem cells from obese mouse and human. PLoS One 10:e0123397 (2015). Read more (PubMed: 25875023) »

    See 1 Publication for this product

    Product Wall

    Both of these kits (ab117133 and ab117135) will immunoprecipitate methylated DNA. The ab117133 Kit uses purified DNA as starting materials while the ab117135 Kit uses cells as starting materials. I can confirm that the immunoprecipitated products are s...

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    Thank you for contacting Abcam.


    This kit can be used for the DNA fragmented by sonication. The kit is tested with various size of DNA and suitable for the size from 150 bp to intact genomic DNA.


    I hope this information i...

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    Thank you for contacting Abcam regarding our EpiSeeker Hydroxymethylated DNA Quantification kits.


    The main difference between the colorimetric and fluorometric kits is the method of detection - this will require either a microplate read...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"