Hydrogen Peroxide Blocking Reagent (ab64218)

製品の概要

  • 製品名Hydrogen Peroxide Blocking Reagent
    Hydrogen peroxide blocking reagent ihc 試薬 製品一覧
  • アプリケーション適用あり: IHC-P, IHC-Frmore details
  • 特記事項The concentration of this product is 0.3%.

    Immunohistochemistry
    Incubate tissue section for 5-10 minutes at room temperature prior to application of the primary antibody. After incubation rinse twice in buffer.

製品の特性

  • 関連性Hydrogen Peroxide Blocking Reagent is intended for use in peroxidase-based immunohistochemical staining procedures on cell preparations, frozen tissue sections, and paraffin-embedded tissue sections.
  • 別名
    • Hydrogen peroxide block

アプリケーション

Our Abpromise guarantee covers the use of ab64218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Hydrogen Peroxide Blocking Reagent (ab64218) 使用論文

ab64218 has not yet been referenced specifically in any publications.

Product Wall

IHC-P with Ki67 antibody using H2O2 blocking reagant

Excellent Excellent 5/5 (Ease of Use)
Abreviews
H2O2 blocking reagant was used prior to protein blocking, and primary antibody was diluted 1:100 and incubated at RT for 1 hr. As the image indicates, the peroxidase activity was significantly blocked and there was minimal background staining.
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Abcam user community

Verified customer

投稿 Aug 16 2016

Hydrogen peroxidase Blocking Solution

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Using HRP conjugated antibody may result in high, non-specific background staining. The non-specific background can be significantly reduced by pre-treatment of tissues with hydrogen peroxide prior to incubation with HRP conjugated antibody. Pictures show mouse gastrocnemius staining with no pre-treatment and pre-treatment with Hydrogen Peroxide Blocking solution from abcam. Results shows a significant improvement with an abolished background staining.
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Dr. FRANCESCO ELIA MARINO

Verified customer

投稿 Jul 23 2014


ab64218 and ab94666 are exactly the same products but ab64218 is sold as 125 ml and ab94666 is sold as 60 ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"