アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Incubate tissue section for 5-10 minutes at room temperature prior to application of the primary antibody. After incubation rinse twice in buffer.
Our Abpromise guarantee covers the use of ab64218 in the following tested applications.
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block (ab64218) to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.