Anti-Hyaluronidase PH20 抗体 (ab22199)

製品の概要

  • 製品名Anti-Hyaluronidase PH20 antibody
    Hyaluronidase PH20 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Hyaluronidase PH20
  • アプリケーション適用あり: WBmore details
  • 種交差性
    Reacts with Streptococcus pyogenes. Not yet tested in other species.
  • 免疫原

    Fusion protein:

    ADTLTSNSEPNNTYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIA GNDAYDKTNPDMVTFHNKAEKDAQNIIKSYQGLDHENR

    , corresponding to amino acids 33/120 of Streptococcus pyogenes Hyaluronidase

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 99 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
  • 組織特異性Testis.
  • 配列類似性Belongs to the glycosyl hydrolase 56 family.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 別名
    • epididymis secretory sperm binding protein Li 96n antibody
    • HEL-S-96n antibody
    • HYA1 antibody
    • HYAL PH-20 antibody
    • HYAL PH20 antibody
    • Hyal-PH20 antibody
    • HYAL1 antibody
    • HYAL3 antibody
    • HYAL5 antibody
    • HYALP_HUMAN antibody
    • Hyaluronidase PH-20 antibody
    • Hyaluronoglucosaminidase PH-20 antibody
    • MGC26532 antibody
    • PH-20 Hyaluronidase antibody
    • PH20 antibody
    • PH20 Hyaluronidase antibody
    • SPAG15 antibody
    • SPAM-1 antibody
    • Spam1 antibody
    • sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) antibody
    • Sperm adhesion molecule 1 antibody
    • Sperm surface protein PH-20 antibody
    • Sperm surface protein PH20 antibody
    • zona pellucida binding antibody
    see all

Anti-Hyaluronidase PH20 antibody 画像

  • All lanes : Anti-Hyaluronidase PH20 antibody (ab22199) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 99 kDa

Anti-Hyaluronidase PH20 antibody (ab22199) 使用論文

ab22199 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-P. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by s...

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Thank you for your enquiry. Ab22199 is suitable for use in Western blotting but has not yet been tested for application in IHC (or any other applications). If you decide to go ahead and purchase this product, please let us know how you get on by sub...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"