製品の概要

  • 製品名Anti-HYAL3 antibody
    HYAL3 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to HYAL3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length HYAL3 protein (Human)

  • ポジティブ・コントロール
    • Hela cells. HYAL3 transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).

    NOTE: This product requires purification with Protein A prior to use in ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells.
    • 配列類似性Belongs to the glycosyl hydrolase 56 family.
      Contains 1 EGF-like domain.
    • 細胞内局在Secreted. Lysosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Hyal-3 antibody
      • HYAL3 antibody
      • HYAL3_HUMAN antibody
      • Hyaluronidase 3 antibody
      • Hyaluronidase-3 antibody
      • Hyaluronoglucosaminidase 3 antibody
      • Hyaluronoglucosaminidase-3 antibody
      • LUCA-3 antibody
      • LUCA14 antibody
      • LUCA3 antibody
      • Lung carcinoma protein 3 antibody
      • Minna14 antibody
      see all

    Anti-HYAL3 antibody 画像

    • All lanes : Anti-HYAL3 antibody (ab67248) at 1/500 dilution

      Lane 1 : HYAL3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
    • Immunofluorescent staining of HYAL3 in HeLa cells using purified ab67248 at 10µg/ml.

    Anti-HYAL3 antibody (ab67248) 使用論文

    ab67248 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (fibroblast)
    Specification fibroblast
    Treatment 10ng/ml IL-1beta 24hrs
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    投稿 Feb 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"