製品の概要

  • 製品名
    Anti-HYAL2 antibody
    HYAL2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HYAL2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HYAL2.

    Immunogen の所有権に関して

    (Peptide available as ab68607.)

  • ポジティブ･コントロール
    • This antibody gave a positive signal in the following lysates: Rat Liver Tissue, Mouse Liver Tissue, JEG-3 Whole Cell, Human Thymus Tissue, HeLa Whole Cell, HepG2 Whole Cell, Raji Whole Cell, SW480 Whole Cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 23365098
IHC-P 1/100. PubMed: 23560496
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human HYAL2 peptide (ab68607).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.
  • 組織特異性
    Widely expressed. No expression detected in adult brain.
  • 配列類似性
    Belongs to the glycosyl hydrolase 56 family.
    Contains 1 EGF-like domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Hyal 2 antibody
    • Hyal-2 antibody
    • Hyal2 antibody
    • HYAL2_HUMAN antibody
    • Hyaluronidase 2 antibody
    • Hyaluronidase 2 Precursor antibody
    • Hyaluronidase-2 antibody
    • Hyaluronoglucosaminidase 2 antibody
    • Hyaluronoglucosaminidase-2 antibody
    • LUCA 2 antibody
    • LUCA-2 antibody
    • LUCA2 antibody
    • Lung carcinoma protein 2 antibody
    • lysosomal hyaluronidase antibody
    • PH 20 homolog antibody
    • PH-20 homolog antibody
    • PH20 homolog antibody
    see all

画像

  • Western blot - Anti-HYAL2 antibody (ab68608)
    Western blot - Anti-HYAL2 antibody (ab68608)
    All lanes : Anti-HYAL2 antibody (ab68608) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Human thymus tissue lysate - total protein (ab30146)
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : Raji whole cell lysate (ab30124)
    Lane 8 : SW480 whole cell lysate (ab3957)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa, 54 kDa (possible isoform), 58 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-HYAL2 antibody (ab68608)
    Immunocytochemistry/ Immunofluorescence - Anti-HYAL2 antibody (ab68608)
    ICC/IF image of ab68608 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68608, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

参考文献

This product has been referenced in:
  • Modelski MJ  et al. Hyaluronidase 2: a novel germ cell hyaluronidase with epididymal expression and functional roles in mammalian sperm. Biol Reprod 91:109 (2014). Read more (PubMed: 25232017) »
  • Siponen M  et al. Altered expression of hyaluronan, HAS1-2, and HYAL1-2 in oral lichen planus. J Oral Pathol Med N/A:N/A (2014). Read more (PubMed: 25421996) »

See all 5 Publications for this product

ab68608 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

4 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-HYAL2 antibody

 Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (fibroblast)
Specification
fibroblast
Treatment
10 ng/ml IL-1beta 24hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Feb 26 2014

Immunoprecipitation abreview for Anti-HYAL2 antibody

 Excellent
Abreviews
Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Mouse Cell lysate - whole cell (sperm)
Specification
sperm
Total protein in input
125 µg
Read More
Username

Dr. Patricia Martin-Deleon

Verified customer

投稿 Feb 21 2014

Hi, I would like to test the following product in the indicated application and/or species noted below:

Anti-HYAL2 antibody (ab68608)

I’d like to try this on immunohistochemistry in sheep. I will use sheep lung tissue, fixed i...

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DISCOUNT CODE: XXXXXXXX
Expiration date: 07-01-2013
Value: £227
I am very pleased to hear you would like to accept our offer and test ab68608 in IHC on sheep samples. This code will give you a discount off your next order before the expir...

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I am interested in buying an antibody to Hyal-2 that works on immunohistochemistry in sheep. I was looking at one of your products (ab68608). As most antibodies are not raised to sheep antigens I usually compare the immunogen sequence with the sheep pr...

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Thank you for contacting us.
I can confirm that the immunogen of ab68608 shows 100% homology with sheep sequence; http://www.uniprot.org/uniprot/Q8SQG7 so the antibody should cross react.
These antibodies are not yet tested in Immunohistochemi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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