製品の概要
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製品名Anti-HYAL2 antibody
HYAL2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HYAL2
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由来種Rabbit
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アプリケーション適用あり: IHC-Fr, IHC-P, WB, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Dog, Pig, Chimpanzee
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HYAL2.
(Peptide available as ab68607.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: Rat Liver Tissue, Mouse Liver Tissue, JEG-3 Whole Cell, Human Thymus Tissue, HeLa Whole Cell, HepG2 Whole Cell, Raji Whole Cell, SW480 Whole Cell
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab68608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 23365098 | |
| IHC-P | 1/100. PubMed: 23560496 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human HYAL2 peptide (ab68607). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.
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組織特異性Widely expressed. No expression detected in adult brain.
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配列類似性Belongs to the glycosyl hydrolase 56 family.
Contains 1 EGF-like domain. -
細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 8692 Human
- Entrez Gene: 15587 Mouse
- Entrez Gene: 404697 Pig
- Entrez Gene: 64468 Rat
- Omim: 603551 Human
- SwissProt: Q12891 Human
- SwissProt: O35632 Mouse
- SwissProt: Q9Z2Q3 Rat
see all -
別名
- Hyal 2 antibody
- Hyal-2 antibody
- Hyal2 antibody
see all
画像
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Western blot - HYAL2 antibody (ab68608)All lanes : Anti-HYAL2 antibody (ab68608) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 :Raji whole cell lysate (ab30124)
Lane 8 :SW480 whole cell lysate (ab3957)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa (why is the actual band size different from the predicted?)
Additional bands at: 40 kDa, 54 kDa (possible isoform), 58 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - HYAL2 antibody (ab68608)ICC/IF image of ab68608 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68608, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
プロトコール
参考文献
This product has been referenced in:
- Modelski MJ et al. Hyaluronidase 2: a novel germ cell hyaluronidase with epididymal expression and functional roles in mammalian sperm. Biol Reprod 91:109 (2014). Read more (PubMed: 25232017) »
- Siponen M et al. Altered expression of hyaluronan, HAS1-2, and HYAL1-2 in oral lichen planus. J Oral Pathol Med N/A:N/A (2014). Read more (PubMed: 25421996) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"