製品の概要

  • 製品名Anti-HYAL2 antibody
    HYAL2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HYAL2
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HYAL2.

    (Peptide available as ab68607.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Rat Liver Tissue, Mouse Liver Tissue, JEG-3 Whole Cell, Human Thymus Tissue, HeLa Whole Cell, HepG2 Whole Cell, Raji Whole Cell, SW480 Whole Cell

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 23365098
IHC-P 1/100. PubMed: 23560496
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human HYAL2 peptide (ab68607).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R.
  • 組織特異性Widely expressed. No expression detected in adult brain.
  • 配列類似性Belongs to the glycosyl hydrolase 56 family.
    Contains 1 EGF-like domain.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Hyal 2 antibody
    • Hyal-2 antibody
    • Hyal2 antibody
    • HYAL2_HUMAN antibody
    • Hyaluronidase 2 antibody
    • Hyaluronidase 2 Precursor antibody
    • Hyaluronidase-2 antibody
    • Hyaluronoglucosaminidase 2 antibody
    • Hyaluronoglucosaminidase-2 antibody
    • LUCA 2 antibody
    • LUCA-2 antibody
    • LUCA2 antibody
    • Lung carcinoma protein 2 antibody
    • lysosomal hyaluronidase antibody
    • PH 20 homolog antibody
    • PH-20 homolog antibody
    • PH20 homolog antibody
    see all

Anti-HYAL2 antibody 画像

  • All lanes : Anti-HYAL2 antibody (ab68608) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Human thymus tissue lysate - total protein (ab30146)
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : Raji whole cell lysate (ab30124)
    Lane 8 : SW480 whole cell lysate (ab3957)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,54 kDa (possible isoform),58 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab68608 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68608, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

Anti-HYAL2 antibody (ab68608) 使用論文

This product has been referenced in:
  • Modelski MJ  et al. Hyaluronidase 2: a novel germ cell hyaluronidase with epididymal expression and functional roles in mammalian sperm. Biol Reprod 91:109 (2014). Read more (PubMed: 25232017) »
  • Siponen M  et al. Altered expression of hyaluronan, HAS1-2, and HYAL1-2 in oral lichen planus. J Oral Pathol Med N/A:N/A (2014). Read more (PubMed: 25421996) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (fibroblast)
Specification fibroblast
Treatment 10 ng/ml IL-1beta 24hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 26 2014

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Mouse Cell lysate - whole cell (sperm)
Specification sperm
Total protein in input 125 µg
Username

Dr. Patricia Martin-DeLeon

Verified customer

投稿 Feb 21 2014

DISCOUNT CODE: XXXXXXXX
Expiration date: 07-01-2013
Value: £227
I am very pleased to hear you would like to accept our offer and test ab68608 in IHC on sheep samples. This code will give you a discount off your next order before the expir...

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Thank you for contacting us.
I can confirm that the immunogen of ab68608 shows 100% homology with sheep sequence; http://www.uniprot.org/uniprot/Q8SQG7 so the antibody should cross react.
These antibodies are not yet tested in Immunohistochemi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"