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RabMAb

Anti-HUS1 抗体 [EPR5132] (ab109371)

製品の概要

  • 製品名
    Anti-HUS1 antibody [EPR5132]
    HUS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5132] to HUS1
  • アプリケーション
    適用あり: WB, IP, IHC-P, Flow Cytmore details
    適用なし: ICC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human HUS1.

  • ポジティブ・コントロール
    • HeLa, K562, and A431 cell lysates, Human breast carcinoma tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for ICC.
  • ターゲット情報

    • 機能
      Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
    • 組織特異性
      Ubiquitous.
    • 配列類似性
      Belongs to the HUS1 family.
    • 細胞内局在
      Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Checkpoint protein HUS1 antibody
      • hHUS1 antibody
      • HUS1 (S. pombe) checkpoint homolog antibody
      • Hus1 antibody
      • HUS1 checkpoint homolog (S. pombe) antibody
      • HUS1 Checkpoint Protein antibody
      • HUS1 protein antibody
      • HUS1+ - like protein antibody
      • HUS1_HUMAN antibody
      • Hydroxyurea-sensitive 1, S. pombe, homolog of antibody
      see all

    画像

    • Overlay histogram showing HeLa cells stained with ab109371 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109371, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-HUS1 antibody [EPR5132] (ab109371) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 32 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109371 at a dilution of 1/100.

    参考文献

    ab109371 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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