Anti-HUS1 抗体 (ab96297)
Key features and details
- Rabbit polyclonal to HUS1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HUS1 antibody
HUS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HUS1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Pig -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 - 243 of Human HUS1 (AAH07013).
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ポジティブ・コントロール
- 293T cell lysate, CA922 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96297の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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IHC-P |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the HUS1 family. -
細胞内局在
Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 514142 Cow
- Entrez Gene: 3364 Human
- Entrez Gene: 100192318 Pig
- Entrez Gene: 498411 Rat
- Omim: 603760 Human
- SwissProt: O60921 Human
- Unigene: 152983 Human
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別名
- Checkpoint protein HUS1 antibody
- hHUS1 antibody
- HUS1 (S. pombe) checkpoint homolog antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab96297 は 4 報の論文で使用されています。
- Lindner AK et al. HUS1 as a Potential Therapeutic Target in Urothelial Cancer. J Clin Med 11:N/A (2022). PubMed: 35456300
- Pillay N et al. DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors. Cancer Cell 35:519-533.e8 (2019). PubMed: 30889383
- Osorio-Zambrano WF & Davey S Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling. PLoS One 10:e0144434 (2015). PubMed: 26658951
- Ding G et al. HIF1-regulated ATRIP expression is required for hypoxia induced ATR activation. FEBS Lett 587:930-5 (2013). PubMed: 23454212