製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HUS1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow, Pig
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 - 243 of Human HUS1 (AAH07013).

  • ポジティブ・コントロール
    • 293T cell lysate, CA922 xenograft

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab96297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the HUS1 family.
  • 細胞内局在
    Nucleus. Cytoplasm. In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Checkpoint protein HUS1 antibody
    • hHUS1 antibody
    • HUS1 (S. pombe) checkpoint homolog antibody
    • Hus1 antibody
    • HUS1 checkpoint homolog (S. pombe) antibody
    • HUS1 Checkpoint Protein antibody
    • HUS1 protein antibody
    • HUS1+ - like protein antibody
    • HUS1_HUMAN antibody
    • Hydroxyurea-sensitive 1, S. pombe, homolog of antibody
    see all

画像

  • Anti-HUS1 antibody (ab96297) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 32 kDa
  • ab96297 at 1/500 dilution, staining HUS1 in paraffin-embedded CA922 xenograft.

参考文献

This product has been referenced in:
  • Osorio-Zambrano WF & Davey S Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling. PLoS One 10:e0144434 (2015). Read more (PubMed: 26658951) »
  • Ding G  et al. HIF1-regulated ATRIP expression is required for hypoxia induced ATR activation. FEBS Lett 587:930-5 (2013). Read more (PubMed: 23454212) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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