製品の概要

  • 製品名Anti-HURP antibody
    HURP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HURP
  • アプリケーション適用あり: IHC-P, WBmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region between residues 200 and 250 of Human HURP (NP_055565.2).

  • ポジティブ・コントロール
    • Human basal cell carcinoma and human seminoma tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
  • 追加情報IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
    WB: 1/200.

    Is unsuitable for ICC/IF.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.
    • 組織特異性Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.
    • 配列類似性Belongs to the SAPAP family.
    • 発生段階Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.
    • 翻訳後修飾Ubiquitinated, leading to its degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR. Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.
    • 細胞内局在Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.
    • Information by UniProt
    • 参照データベース
    • 別名
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    Anti-HURP antibody 画像

    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human basal cell carcinoma by Immunohistochemistry, using DAB staining.
    • ab84509, at 1/250 dilution, staining HURP in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry, using DAB staining.
    • Anti-HURP antibody (ab84509) at 1/200 dilution + 25µg of WCE from U20S cells synchronized by thimidine arrest and release to enrich in mitotic cells (same on lane 1 and 2).

    Anti-HURP antibody (ab84509) 使用論文

    This product has been referenced in:
    • Ma MP  et al. Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. PLoS One 8:e68387 (2013). Read more (PubMed: 23861900) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"