製品の概要

  • 製品名
    Anti-HuR / ELAVL1 antibody
    HuR / ELAVL1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to HuR / ELAVL1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: MSNGYEDHMA EDCRGDIGRT NLIVNYLPQN MTQDELRSLF SSIGEVESAK LIRDKVAGHS LGYGFVNYVT AKDAERAINT LNGLRLQSKT IKVSYARPSS , corresponding to amino acids 1-101 of Human HuR/ ELAVL1

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
    • 組織特異性
      Ubiquitous.
    • 配列類似性
      Belongs to the RRM elav family.
      Contains 3 RRM (RNA recognition motif) domains.
    • 翻訳後修飾
      Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
      • ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
      • ELAV like 1 antibody
      • ELAV like RNA binding protein 1 antibody
      • ELAV-like protein 1 antibody
      • ELAV1 antibody
      • ELAV1_HUMAN antibody
      • Elavl1 antibody
      • Embryonic lethal abnormal vision drosophila homolog like 1 antibody
      • Hu Antigen R antibody
      • Hu-antigen R antibody
      • Hua antibody
      • HuR antibody
      • MelG antibody
      see all

    画像

    • ab54987 Immunoprecipitating HuR / ELAVL1 in Human lymphocyte cell lysate. 700µg of cell lysate was incubated with primary antibody (5 µg/mg in abcam non-denaturing buffer) and matrix (Protein G) for 2 hours at 4°C. For western blot, ab54987 (1/1000) was used to confirm successful immunoprecipitation. Lane 1 - 25μg of lysate pre-IP, Lanes 2 and 3 -15μg of 70μg elution from matrix. 4-12% Bis-Tris gel.

      See Abreview



    • Predicted band size : 36 kDa
      HuR / ELAVL1 antibody (ab54987) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    参考文献

    This product has been referenced in:
    • Chai Y  et al. HuR-regulated lncRNA NEAT1 stability in tumorigenesis and progression of ovarian cancer. Cancer Med 5:1588-98 (2016). WB ; Human . Read more (PubMed: 27075229) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (lymphocytes)
    Specification
    lymphocytes
    Total protein in input
    700 µg
    Username

    Abcam user community

    Verified customer

    投稿 Aug 30 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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