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Our Abpromise guarantee covers the use of ab46174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cryostat section of the rat spinal cord was incubated in ab46174 to the rat HAP1 protein at the dilution of 1:1000 overnight followed with a biotinylated secondary antibody and ABC kit. The section was developed in diaminobenzidine solution and enhanced in the presence of nickel sulphate. Cell bodies and nerve fibers in the spinal cord nerve are stained.
ab46174 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"