Human Wilms Tumor Protein peptide (ab73466)



  • 由来
  • アミノ酸配列
    • 生物種


Our Abpromise guarantee covers the use of ab73466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション


  • 精製度
    70 - 90% by HPLC.

  • 製品の状態
  • 備考

    This is the blocking peptide for ab64681.

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...


  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.


  • 別名
    • WIT 2
    • WT 1
    • AWT1
    • FWT1
    • GUD
    • NPHS4
    • WAGR
    • Wilms Tumor
    • Wilms tumor 1
    • Wilms tumor protein
    • Wilms' tumor gene
    • Wilms' tumor protein
    • WIT2
    • WT
    • WT1
    • WT1_HUMAN
    • WT33
    see all
  • 機能
    Transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • 組織特異性
    Expressed in the kidney and a subset of hematopoietic cells.
  • 関連疾患
    Defects in WT1 are the cause of Frasier syndrome (FS) [MIM:136680]. FS is characterized by a slowly progressing nephropathy leading to renal failure in adolescence or early adulthood, male pseudohermaphroditism, and no Wilms tumor. As for histological findings of the kidneys, focal glomerular sclerosis is often observed. There is phenotypic overlap with Denys-Drash syndrome. Inheritance is autosomal dominant.
    Defects in WT1 are the cause of Wilms tumor 1 (WT1) [MIM:194070]. WT is an embryonal malignancy of the kidney that affects approximately 1 in 10'000 infants and young children. It occurs both in sporadic and hereditary forms.
    Defects in WT1 are the cause of Denys-Drash syndrome (DDS) [MIM:194080]. DDS is a typical nephropathy characterized by diffuse mesangial sclerosis, genital abnormalities, and/or Wilms tumor. There is phenotypic overlap with WAGR syndrome and Frasier syndrome. Inheritance is autosomal dominant, but most cases are sporadic.
    Defects in WT1 are the cause of nephrotic syndrome type 4 (NPHS4) [MIM:256370]. A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. Most patients with NPHS4 show diffuse mesangial sclerosis on renal biopsy, which is a pathologic entity characterized by mesangial matrix expansion with no mesangial hypercellularity, hypertrophy of the podocytes, vacuolized podocytes, thickened basement membranes, and diminished patency of the capillary lumen.
    Defects in WT1 are a cause of Meacham syndrome (MEACHS) [MIM:608978]. Meacham syndrome is a rare sporadically occurring multiple malformation syndrome characterized by male pseudohermaphroditism with abnormal internal female genitalia comprising a uterus and double or septate vagina, complex congenital heart defect and diaphragmatic abnormalities.
    Note=A chromosomal aberration involving WT1 may be a cause of desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with EWSR1.
  • 配列類似性
    Belongs to the EGR C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm; Nucleus > nucleoplasm and Nucleus speckle.
  • Information by UniProt


ab73466 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your reply. To ensure that your message gets directed to the person who has been handling your case, please do not alter the subject line when replying to our emails. This will allow your case to be tracked effectively in our system.
Read More

Thank you for your patience in this matter. I have processed the request for you to receive ab64681 as a replacement for ab15249 which did not work in western blot as stated on the datasheet. I have requested that the peptide ab73466 be included in the...

Read More

Thank you for sending a copy of the original order for ab15249. The order only shows that 1 vial of ab15249 was ordered, could you send me the order for the second vial please. I can process the request for 1 replacement vial now so that you can get it...

Read More

Thank you for your reply. I will be happy to send the peptide ab73466 free of charge with your replacement antibody. We do not have any western blot images for ab15249 and so I am unbale to send you any images as you requested. I look forward to your r...

Read More

Thank you for your reply. I would be very happy to send you ab64681 as a replacement for ab15249, which did not work as guaranteed in western blot. To process your replacement as quickly as possible could you please send me the original order details f...

Read More

Thank you for your inquiry. Unfortunately, ab73466 (Wilms Tumor Protein peptide) is unsuitable as blocking peptide for ab15249 (Anti-Wilms Tumor Protein antibody). But I am happy to confirm that ab73466 (Wilms Tumor Protein peptide) was used as immunog...

Read More