製品の概要

  • 製品名
    Human VEGFC ELISA Kit
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    13.72 pg/ml - 10000 pg/ml
  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 90.46 83% - 99%
    Serum 109.4 100% - 118%
    Plasma 96.02 86% - 104%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s VEGFC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGFC in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human VEGFC coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGFC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGFC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab100664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative standard curve using ab100664

  • Representative standard curve using ab100664

プロトコール

参考文献

ab100664 has not yet been referenced specifically in any publications.

レビューと Q&A

Vielen Dank für Ihren Anruf.

Leider ist es so, wie ich befürchtet habe, wir können leider keine Angabe zu diesen Kits mit HepG2 Zellen, bzw mit anderen Leberzelllinien machen. Grundsätzlich spricht aber nichts gegen die Ver...

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The capture antibody used in this kit is monoclonal and detects human VEGF-C in ELISAs and Western blots. In direct ELISAs, this antibody does not cross­react with recombinant human (rh)PDGF­AA, rhPDGF­...

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Thank you for your enquiry.

ab100664 VEGFC Human ELISA Kit :

I can confirm this kit recognizes the mature form of VEGF-C (aa 103-227). Based on sequence similarity, the kit is also expected to detect the pro-form but this has not ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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