製品の概要

  • 製品名Human VEGF Receptor 3 ELISA Kit
    VEGF Receptor 3 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    12.29 pg/ml - 3000 pg/ml
  • 添加回収試験

    96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 97.35 89% - 106%
    Serum 95.46 86% - 104%
    Plasma 96.47 88% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s VEGF Receptor 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF Receptor 3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human VEGF Receptor 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF Receptor 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.
  • 細胞内局在Cell membrane; Single-pass type I membrane protein.
  • 別名
    • FLT 4
    • FLT4
    • FLT41
    • Fms like tyrosine kinase 4
    • Fms related tyrosine kinase 4
    • LMPH1A
    • PCL
    • Tyrosine protein kinase receptor FLT4
    • Vascular endothelial growth factor receptor 3
    • VEGFR 3
    • VEGFR3
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human VEGF Receptor 3 ELISA Kit 画像

  • Hu VEGF receptor 3 measured in biofluids showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100666

  • Representative standard curve using ab100666

プロトコール

Human VEGF Receptor 3 ELISA Kit (ab100666) 使用論文

ab100666 has not yet been referenced specifically in any publications.

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