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|Cell culture supernatant||100||86% - 107%|
|Serum||109||107% - 110%|
|Heparin Plasma||105||104% - 107%|
|EDTA Plasma||83||81% - 84%|
|Citrate Plasma||80||78% - 81%|
VEGF R1 (FLT1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VEGF R1 protein in Human serum, plasma and cell culture supernatant samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
VEGF R1 is a tyrosine protein kinase receptor that is essential for embryonic vasculature development, angiogenesis, cell survival, cell migration, chemotaxis, cancer cell invasion, and macrophage function. VEGF R1 can homodimerize or hererodimerize with VEGF R2, and acts as a receptor for VEGFA, VEGFB, and PGF. VEGF R1 is present as a full length, membrane form and as a truncated, soluble receptor that can also sequester ligand or dimerize with full length receptor and prevent signal transduction.
|内容||1 x 96 tests|
|10X VEGF R1 Capture Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|ab221825 - Antibody Diluent 5BI||1 x 6ml|
|Plate Seals||1 unit|
|Sample Diluent 50BS||1 x 20ml|
|Sample Diluent NS (ab193972)||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
|VEGF R1 Detector Antibody (Lyophilized)||2 vials|
|VEGF R1 Human Lyophilized Recombinant Protein||2 vials|
Our Abpromise guarantee covers the use of ab195210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab195210 has not yet been referenced specifically in any publications.