製品の概要

  • 製品名Human VEGF R1 ELISA Kit (FLT1)
    VEGF Receptor 1 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5 6.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 10.3%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    146 pg/ml
  • 検出範囲
    0.391 ng/ml - 25 ng/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 100 86% - 107%
    Serum 109 107% - 110%
    Heparin Plasma 105 104% - 107%
    EDTA Plasma 83 81% - 84%
    Citrate Plasma 80 78% - 81%

  • 全工程の試験時間
    1h 30m
  • ステップOne step assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s VEGF R1 (FLT1) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VEGF R1 protein in Human serum, plasma and cell culture supernatant samples.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 特記事項

    VEGF R1 is a tyrosine protein kinase receptor that is essential for embryonic vasculature development, angiogenesis, cell survival, cell migration, chemotaxis, cancer cell invasion, and macrophage function. VEGF R1 can homodimerize or hererodimerize with VEGF R2, and acts as a receptor for VEGFA, VEGFB, and PGF. VEGF R1 is present as a full length, membrane form and as a truncated, soluble receptor that can also sequester ligand or dimerize with full length receptor and prevent signal transduction.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • 組織特異性Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • FLT
    • FLT 1
    • Flt-1
    • FLT1
    • Fms like tyrosine kinase 1
    • Fms related tyrosine kinase 1
    • Fms-like tyrosine kinase 1
    • Tyrosine protein kinase FRT
    • Tyrosine protein kinase receptor FLT
    • Tyrosine-protein kinase FRT
    • Tyrosine-protein kinase receptor FLT
    • Vascular endothelial growth factor receptor 1
    • Vascular endothelial growth factor/vascular permeability factor receptor
    • Vascular permeability factor receptor
    • VEGFR 1
    • VEGFR-1
    • VEGFR1
    • VGFR1_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab195210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human VEGF R1 ELISA Kit (FLT1) 画像

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

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Human VEGF R1 ELISA Kit (FLT1) (ab195210) 使用論文

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