製品の概要

  • 製品名Human VEGF R1 ELISA Kit (FLT1)
    VEGF Receptor 1 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6 5.5%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6 5.1%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.03 ng/ml
  • 検出範囲
    0.16 ng/ml - 10 ng/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 47% - 79%
    Serum 46% - 67%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s VEGF R1 (FLT1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF R1 (FLT1) in cell culture supernatant, serum and plasma (EDTA, citrate).


    VEGF R1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated VEGF R1 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of VEGF R1 captured on the plate.


    Save time with a 90-minute ELISA protocol. Try Human VEGF R1 ELISA Kit (FLT1) (ab195210) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • 組織特異性Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. It is not expressed in tumor cell lines. Isoform sFlt1 is strongly expressed in placenta.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • FLT
    • FLT 1
    • Flt-1
    • FLT1
    • Fms like tyrosine kinase 1
    • Fms related tyrosine kinase 1
    • Fms-like tyrosine kinase 1
    • Tyrosine protein kinase FRT
    • Tyrosine protein kinase receptor FLT
    • Tyrosine-protein kinase FRT
    • Tyrosine-protein kinase receptor FLT
    • Vascular endothelial growth factor receptor 1
    • Vascular endothelial growth factor/vascular permeability factor receptor
    • Vascular permeability factor receptor
    • VEGFR 1
    • VEGFR-1
    • VEGFR1
    • VGFR1_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human VEGF R1 ELISA Kit (FLT1) 画像

  • Representative Standard Curve using ab119567.

プロトコール

Human VEGF R1 ELISA Kit (FLT1) (ab119567) 使用論文

This product has been referenced in:
  • Wihastuti TA  et al. Vasa vasorum anti-angiogenesis through H2O2, HIF-1a, NF-?B, and iNOS inhibition by mangosteen pericarp ethanolic extract (Garcinia mangostana Linn) in hypercholesterol-diet-given Rattus norvegicus Wistar strain. Vasc Health Risk Manag 10:523-31 (2014). Read more (PubMed: 25187725) »

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