Human VCAM1 ELISA Kit (CD106) (ab46118)

製品の概要

  • 製品名Human VCAM1 ELISA Kit (CD106)
    VCAM1 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 48.79ng/ml 0.221 0.45%
    B 8 5.81ng/ml 0.132 2.27%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 28 49.69ng/ml 0.72 1.44%
    B 28 5.95ng/ml 0.354 5.94%
  • テスト
    2 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.6 ng/ml
  • 検出範囲
    1.56 ng/ml - 50 ng/ml
  • 全工程の試験時間
    1h 40m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human VCAM1 (CD106) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of VCAM1 in cell culture supernatants, buffered solutions, serum, plasma, and other body fluids.

    A monoclonal antibody specific for VCAM1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known VCAM1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for VCAM1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of VCAM1 present in the samples.

    This kit will recognize both endogenous and recombinant Human VCAM1.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 組織特異性Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 配列類似性Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメインEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻訳後修飾Sialoglycoprotein.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD106
    • CD106 Antigen
    • INCAM 100
    • INCAM-100
    • L1CAM
    • MGC99561
    • V-CAM 1
    • Vascular Cell Adhesion Molecule 1
    • Vascular cell adhesion protein 1
    • VCAM 1
    • VCAM-1
    • VCAM1
    • VCAM1_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human VCAM1 ELISA Kit (CD106) 画像

  • Representative Standard Curve using ab46118.

プロトコール

Human VCAM1 ELISA Kit (CD106) (ab46118) 使用論文

ab46118 has not yet been referenced specifically in any publications.

Product Wall

VCAM1 concentration in human plasma

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Human plasma samples were diluted 50 times by diluent buffer.
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Verified customer

投稿 Jun 18 2013

Thank you for contacting us. The laboratory that developed and validated the kit uses linear regression but there are some who argue that 4 parameter logisitic nonlinear regression is a better way to curve-fit ELISA data. Either formula is applicab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"