Human VCAM-1 ELISA Set (without plates) (ab47355)

製品の概要

  • 製品名Human VCAM-1 ELISA Set (without plates)
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.6 ng/ml
  • 検出範囲
    1.56 ng/ml - 50 ng/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human VCAM1

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームReagents

製品の特性

  • 関連性VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.
  • 細胞内局在Cell Membrane; single-pass type I membrane protein
  • 別名
    • CD106
    • CD106 antigen
    • INCAM 100
    • L1CAM
    • Vascular cell adhesion molecule 1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab47355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human VCAM-1 ELISA Set (without plates) 画像

  • VCAM-1 Standard curve ranging from 1.56 to 50 ng/mL

Human VCAM-1 ELISA Set (without plates) (ab47355) 使用論文

ab47355 has not yet been referenced specifically in any publications.

Product Wall

Thanks for your enquiry.

Please find below additional info required for ab47355 and ab47361


1) PBS is prepared as followed : 0.137mM NaCl; 1.47 KH2PO4; 8.1mM Na2PO4, 2H2O

2) Samples should be diluted : 1/100 to 1/2...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"