Human Urokinase type Plasminogen Activator ELISA Kit (ab108917)

製品の概要

  • 製品名Human Urokinase type Plasminogen Activator ELISA Kit
    Urokinase type Plasminogen Activator キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Tissue
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.2 ng/ml
  • 検出範囲
    0.25 ng/ml - 8 ng/ml
  • 添加回収試験

    98.5 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts.

     

    A Urokinase type Plasminogen Activator specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Urokinase type Plasminogen Activator specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Urokinase type Plasminogen Activator captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • 組織特異性Expressed in the prostate gland and prostate cancers.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • ATF
    • BDPLT5
    • Plasminogen activator urinary
    • PLAU
    • QPD
    • u PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-type plasminogen activator chain B
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Urokinase type Plasminogen Activator ELISA Kit 画像

  • Representative Standard Curve using ab108917.

プロトコール

Human Urokinase type Plasminogen Activator ELISA Kit (ab108917) 使用論文

ab108917 has not yet been referenced specifically in any publications.

Product Wall



Both ab108914 and ab108917 contain 12, 8 well strips.


You are correct that you should be able to detect tPA and uPA from cell culture supernatants or cell homogenates with both these kits.

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.
Read More

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"