製品の概要

  • 製品名Human uPAR ELISA Kit (URKR)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 4 pg/ml
  • 検出範囲
    62.5 pg/ml - 4000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human uPAR (URKR) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPAR (URKR) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

    A uPAR (URKR) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPAR (URKR) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA Receptor amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • 組織特異性Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • 配列類似性Contains 3 UPAR/Ly6 domains.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • CD87
    • Monocyte activation antigen Mo3
    • PLAUR
    • U-PAR
    • uPAR
    • UPAR_HUMAN
    • Urokinase plasminogen activator surface receptor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human uPAR ELISA Kit (URKR) 画像

  • Representative standard curve using ab119612

プロトコール

Human uPAR ELISA Kit (URKR) (ab119612) 使用論文

ab119612 has not yet been referenced specifically in any publications.

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