Human uPA Receptor/U-PAR ELISA Kit (URKR) (ab119612)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human uPA Receptor/U-PAR ELISA Kit (URKR)
uPA Receptor/U-PAR キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 4 pg/ml -
検出範囲
62.5 pg/ml - 4000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human uPA Receptor/U-PAR (URKR) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPA Receptor/U-PAR (URKR) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
A uPA Receptor/U-PAR (URKR) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPA Receptor/U-PAR(URKR) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA Receptor amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human uPA Receptor antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human uPA Receptor antibody 1 x 100µl Lyophilized recombinant Human uPA Receptor standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究分野
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機能
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
組織特異性
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
配列類似性
Contains 3 UPAR/Ly6 domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- CD 87
- CD87
- CD87 antigen
see all -
参照データベース
- Entrez Gene: 5329 Human
- Omim: 173391 Human
- SwissProt: Q03405 Human
- Unigene: 466871 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab119612 は 2 報の論文で使用されています。
- Nantajit D et al. Prognostic significance of uPA and uPAR expression in patients with cervical cancer undergoing radiotherapy. Oncol Lett 21:423 (2021). PubMed: 33850564
- Hamie L et al. SuPAR, a potential inflammatory mediator in psoriasis pathogenesis. Clin Exp Pharmacol Physiol 47:1705-1712 (2020). PubMed: 32558953