製品の概要

  • 製品名Human uPA ELISA Kit (URK)
    Urokinase type Plasminogen Activator キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    62.5 pg/ml - 4000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human uPA (URK) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPA in cell culture supernatants, serum and plasma (heparin, EDTA).

    A uPA specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPA is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA amount of sample captured in plate.

  • 特記事項

    Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • 組織特異性Expressed in the prostate gland and prostate cancers.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • ATF
    • BDPLT5
    • Plasminogen activator urinary
    • PLAU
    • QPD
    • u PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-type plasminogen activator chain B
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human uPA ELISA Kit (URK) 画像

  • Representative standard curve using ab119611

プロトコール

Human uPA ELISA Kit (URK) (ab119611) 使用論文

ab119611 has not yet been referenced specifically in any publications.

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