Human Triiodothyronine ELISA Kit (T3) (ab108663)

製品の概要

  • 製品名
    Human Triiodothyronine ELISA Kit (T3)
    Triiodothyronine (T3) キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Low 20 1.37pg/ml 0.16 11.9%
    Normal 20 4.21pg/ml 0.17 4.1%
    High 20 7.1pg/ml 0.17 2.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Low 10 1.4pg/ml 0.15 10.7%
    Normal 10 4.4pg/ml 0.23 5.2%
    High 10 7pg/ml 0.3 4.2%
  • サンプルの種類
    Serum
  • アッセイタイプ
    Competitive
  • 検出範囲
    0.9 pg/ml - 19 pg/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.

    A 96-well plate has been pre-coated with Anti-Triiodothyronine antibodies. Samples, standards and the Triiodothyronine HRP Conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

  • アプリケーション
    適用あり: Competitive ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

画像

  • Representative standard curve using ab108663

プロトコール

参考文献

ab108663 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
This ELISA kit was used with cell supernatant and the cell extract of HEK293 after homogenisation. The cells were transfected with Type-II Deiodinase variants and were later treated with a T4 medium at a concentration of 20pM. The kit was successful in measuring the concentration of T3 in both the supernatant and cell extract yielding a range of results from 2.9-8.6pg/mL.
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投稿 Jan 06 2016

Abreviews
This kit is not sensitive enough to detect any differences in free T3 generated by cells in cell culture supernatant. It can easily detect exogenous T3 added at 20 nM with strong signal.
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投稿 Oct 30 2015

application to mouse

Good Good 4/5 (Ease of Use)
Abreviews
It seems that mouse serum can be used with this kit, yielding values in the range of 2-6 pg/mL, which is in the range of previously reported values for human free T3 (http://www.globalrph.com/labs_t.htm#T4_(thyroxine)_Total_) and mouse free T3 (http://genesdev.cshlp.org/content/13/10/1329.full - see figure 2A, WT).
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投稿 Sep 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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