製品の概要

  • 製品名Human Transferrin ELISA Kit
    Transferrin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.6%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    = 0.09 µg/ml
  • 検出範囲
    0.098 µg/ml - 25 µg/ml
  • 添加回収試験

    99 %

  • 全工程の試験時間
    2h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Transferrin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in plasma and serum samples.

    A Transferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Transferrin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Transferrin captured in plate.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Apotransferrin
    • Beta 1 metal binding globulin
    • Beta-1 metal-binding globulin
    • DKFZp781D0156
    • PRO1400
    • PRO1557
    • PRO2086
    • Serotransferrin
    • Serotransferrin precursor
    • Siderophilin
    • TF
    • TFQTL1
    • Transferin
    • Transferrin
    • TRFE_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

Human Transferrin ELISA Kit 画像

  • Representative Standard Curve using ab108911.

プロトコール

Human Transferrin ELISA Kit (ab108911) 使用論文

This product has been referenced in:
  • Gomes JA  et al. Brain iron metabolism and brain injury following subarachnoid hemorrhage: iCeFISH-pilot (CSF iron in SAH). Neurocrit Care 21:285-93 (2014). ELISA ; Human . Read more (PubMed: 24710655) »
  • Yu SY  et al. Sleep disorders in Parkinson's disease: clinical features, iron metabolism and related mechanism. PLoS One 8:e82924 (2013). Sandwich ELISA ; Human . Read more (PubMed: 24376607) »

See all 2 Publications for this product

Product Wall



The transferring kit contains two antibodies against full length recombinant protein and purified proteins. Human IgG3 kit contains two antibodies against full length purified proteins.

I am afraid we do not map the epitopes of thes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"