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Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in urine, milk saliva, CSF and cell culture supernatants.
A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.
Get results in 90 minutes with Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.
|内容||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Transferrin Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
|Transferrin Microplate (12 x 8 well strips)||1 unit|
|Transferrin Standard||1 vial|
Our Abpromise guarantee covers the use of ab108902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|