Human Transferrin ELISA Kit (ab108902)

製品の概要

  • 製品名Human Transferrin ELISA Kit
    Transferrin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 1.5 ng/ml
  • 検出範囲
    1.563 ng/ml - 100 ng/ml
  • 添加回収試験

    96.5 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in urine, milk saliva, CSF and cell culture supernatants.


    A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Atransferrinemia
  • 配列類似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • ATRAF
    • Atransferrinemia
    • Beta-1 metal-binding globulin
    • PRO1400
    • Serotransferrin
    • Siderophilin
    • TF
    • Transferrin
    • TRFE_HUMAN
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Transferrin ELISA Kit 画像

  • Representative Standard Curve using ab108902.

プロトコール

Human Transferrin ELISA Kit (ab108902) 使用論文

ab108902 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience in awaiting our reply in the matter of compaibility with the kit. It is difficulty to know whether this will work. If the conjugation block the antibody binding site, it will interfere the assay. If not, it will be fine. ...

Read More

Abreview for Transferrin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2)
Specification: HepG2
Amount of Sample: 0.1ug standard µg
Type of Kit Used: ELISA
Positive control: Provided transferrin standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human transferrin. Did not recognize bovine transferrin in FBS.
Username

Abcam user community

Verified customer

投稿 Jul 04 2012

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"