Human Transferrin ELISA Kit (ab108902)
製品の概要
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製品名Human Transferrin ELISA Kit
Transferrin キット 製品一覧 -
検出方法Colorimetric
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再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 3.7% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.9% -
サンプルの種類Cell culture supernatant, Saliva, Milk
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アッセイタイプSandwich (quantitative)
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検出感度= 1 ng/ml
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検出範囲1.563 ng/ml - 100 ng/ml
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添加回収試験
97 %
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全工程の試験時間4h 00m
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ステップMultiple steps standard assay
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種交差性交差種: Human
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製品の概要
Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in urine, milk saliva, CSF and cell culture supernatants.
A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.
Get results in 90 minutes with Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームMicroplate
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Transferrin Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Transferrin Microplate (12 x 8 well strips) 1 unit Transferrin Standard 1 vial -
研究分野
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機能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
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組織特異性Expressed by the liver and secreted in plasma.
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関連疾患Atransferrinemia
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配列類似性Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
細胞内局在Secreted.
- Information by UniProt
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別名
- ATRAF
- Atransferrinemia
- Beta-1 metal-binding globulin
see all -
参照データベース
- Entrez Gene: 7018 Human
- Omim: 209300 Human
- SwissProt: P02787 Human
- Unigene: 518267 Human
関連製品
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SimpleStep ELISA kits
アプリケーション
Our Abpromise guarantee covers the use of ab108902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Typical Standard CurveRepresentative Standard Curve using ab108902.
プロトコール
参考文献
This product has been referenced in:
- Mehta SR et al. Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment. J Neuroinflammation 14:72 (2017). ELISA ; Human . Read more (PubMed: 28359324) »
- Patton SM et al. Cerebrospinal fluid (CSF) biomarkers of iron status are associated with CSF viral load, antiretroviral therapy, and demographic factors in HIV-infected adults. Fluids Barriers CNS 14:11 (2017). ELISA ; Human . Read more (PubMed: 28427421) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"