製品の概要

  • 製品名Human TNFRSF18 ELISA Kit (GITR)
    TNFRSF18 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    13.7 pg/ml - 10000 pg/ml
  • 添加回収試験

    94 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.69 84% - 105%
    Serum 94.18 83% - 104%
    Plasma 92.75 82% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TNFRSF18 (GITR) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNFRSF18 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human TNFRSF18 coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFRSF18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFRSF18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells.
  • 別名
    • Activation inducible TNFR family receptor
    • AITR
    • GITR
    • GITR D
    • Glucocorticoid induced TNFR related protein
    • Tumor necrosis factor receptor superfamily member 18
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human TNFRSF18 ELISA Kit (GITR) 画像

  • GITR measured in PBMC lysates (RIPA; 0.02-0.1 mg of proteins tested per well) after 72 hours of culture (1e6 cells per mL) in 0.1% DMSA (CTRL), 5 ug x mL-1 PHA (Sigma) or in 50ng x mL-1 PMA (ab120297) with 1uM ionomycin (ab120116) (P/I) (duplicates +/- SD).

  • GITR measured in undiluted human biological fluids (undiluted; duplicates +/- SD).

  • Standard curve in diluent B, with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100527

  • Standard curve in diluent A, with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100527

プロトコール

Human TNFRSF18 ELISA Kit (GITR) (ab100527) 使用論文

ab100527 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab100527.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"