製品の概要

  • 製品名Human TNF beta ELISA Kit
    TNF beta キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3 8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3 10.2%
  • テスト
    1 x 96 test
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    4.6 pg/ml
  • 検出範囲
    15.6 pg/ml - 1000 pg/ml
  • 添加回収試験

    105 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 105 87% - 130%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TNF beta Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TNF beta concentrations in cell culture supernatant, serum and plasma.

    TNF beta specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with HRP-conjugated TNF beta detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is the added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the TNF beta amount of sample captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • 関連疾患Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 細胞内局在Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
  • Information by UniProt
  • 別名
    • LTalpha
    • DIF
    • LT
    • LT alpha
    • LT-alpha
    • Lta
    • Lymphotoxin alpha
    • Lymphotoxin alpha (TNF superfamily, member 1)
    • Lymphotoxin-alpha
    • TNF B
    • TNF superfamily member 1
    • TNF, lymphocyte-derived
    • TNF-beta
    • TNFB
    • TNFB_HUMAN
    • TNFbeta
    • TNFSF1
    • TNLG1E
    • Tumor necrosis factor beta
    • tumor necrosis factor ligand 1E
    • Tumor necrosis factor ligand superfamily member 1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human TNF beta ELISA Kit 画像

  • Representative Standard Curve using ab119561.

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Human TNF beta ELISA Kit (ab119561) 使用論文

ab119561 has not yet been referenced specifically in any publications.

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