Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904)

製品の概要

  • 製品名Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI)
    Tissue Factor Pathway Inhibitor キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.4%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Milk, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    > 0.12 ng/ml
  • 検出範囲
    0.313 ng/ml - 20 ng/ml
  • 添加回収試験

    96.5 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.

    A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 組織特異性Mostly in endothelial cells.
  • 配列類似性Contains 3 BPTI/Kunitz inhibitor domains.
  • ドメインThis inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Secreted and Microsome membrane.
  • Information by UniProt
  • 別名
    • EPI
    • Extrinsic pathway inhibitor
    • LACI
    • Lipoprotein-associated coagulation inhibitor
    • TFPI
    • TFPI1_HUMAN
    • Tissue factor pathway inhibitor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) 画像

  • TFPI measured in various samples showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab108904.

プロトコール

Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904) 使用論文

ab108904 has not yet been referenced specifically in any publications.

Product Wall

We can confirm that heparin does not interfere with the assay. However, we are not sure about protamine. This has not been tested before.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"