製品の概要

  • 製品名
    Human Tissue Factor ELISA Kit (CD142)
    Tissue Factor キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7%
  • サンプルの種類
    Serum, Plasma, Cell culture extracts, Tissue
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 20 pg/ml
  • 検出範囲
    12.5 pg/ml - 400 pg/ml
  • 添加回収試験

    97.5 %

  • 全工程の試験時間
    5h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Tissue Factor (CD142) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor concentrations in plasma, serum, tissue extracts and cell culture supernatants.

    A Tissue Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

画像

  • CD142 measured in cell lysates showing quantity (pg) per 1 million cells tested

  • CD142 measured in various biofluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab108903.

プロトコール

参考文献

This product has been referenced in:
  • Park MY  et al. Biomarkers of kidney injury and klotho in patients with atherosclerotic renovascular disease. Clin J Am Soc Nephrol 10:443-51 (2015). Sandwich ELISA ; Human . Read more (PubMed: 25542906) »

See 1 Publication for this product

レビューと Q&A

The number of cells used per sample with ab108903 Human Tissue Factor ELISA Kit (CD142) will very much depend on the amount of TF in the specific sample being tested.  If the expression level is low, you might want to try 1 million cell per sample (per...

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Thank you for contacting us.

Please attach the results and send us as per your earliest convenience. It will be a great help if could send the protocol exactly as used in experiment.

I hope this information is helpful to you. Please d...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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