Human Tissue Factor ELISA Kit (CD142) (ab108903)


  • 製品名Human Tissue Factor ELISA Kit (CD142)
    Tissue Factor キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 4.9%
    サンプル N 平均値 SD CV%
    Overall 7%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma, Cell culture extracts, Tissue
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 20 pg/ml
  • 検出範囲
    12.5 pg/ml - 400 pg/ml
  • 添加回収試験

    97.5 %

  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Tissue Factor (CD142) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor concentrations in plasma, serum, tissue extracts and cell culture supernatants.

    A Tissue Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 配列類似性Belongs to the tissue factor family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 別名
    • CD142
    • CD142 antigen
    • Coagulation factor III
    • Coagulation factor III (thromboplastin tissue factor)
    • F3
    • FLJ17960
    • TF
    • TF_HUMAN
    • TFA
    • Thromboplastin
    • Tissue factor
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab108903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Tissue Factor ELISA Kit (CD142) 画像

  • CD142 measured in cell lysates showing quantity (pg) per 1 million cells tested

  • CD142 measured in various biofluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab108903.


Human Tissue Factor ELISA Kit (CD142) (ab108903) 使用論文

ab108903 has not yet been referenced specifically in any publications.

Product Wall

Testing crossreactivity in Rhesus EDTA plasma

Good Excellent 5/5 (Ease of Use)
The Tissue Factor ELISA, using the Abcam human ELISA kit, was performed by an experienced laboratory technician at an accredited university. The goal of the experiment was to analyze the linearity of dilutions of rhesus macaque EDTA-prepared plasma samples, but more specifically, to analyze the cross reactivity for rhesus macaque.

Overall, the kit, like other Abcam ELISA kits, was easy to follow, clear in terms of directions, and brief yet thorough. The laboratory technician had no problems understanding any of the protocol’s steps.

Our experiment consisted of using EDTA-prepared Rhesus Macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma was diluted to several different dilutions, as well as the suggested dilution from the kit (1:2, 1:4, 1:8, and 1:16) in the given diluent. The rest of the assay was performed per the protocol’s instructions.

Absorbance measurements of the diluted samples were very comparable between duplicates however, there was a clear lack of linearity between all dilutions’ Tissue Factor value for each sample (see image).

The standard curve was clean with a good range of ODs (see image).

The technician also performed a spike recovery, and found that, between pure diluent with known standard and plasma with known standard, plasma masked the known standard’s concentration in detection using this assay. A spiked concentration of 50 pg/mL was added to pure plasma, then the sample was diluted the same as unspiked sample. Resultant values were the same as unspiked plasma, i.e. we could not detected the spiked protein in rhesus plasma.

Overall, we do not recommend this kit for detection of Tissue Factor in EDTA-prepared Rhesus macaque plasma samples.

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投稿 Jun 01 2016

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Please attach the results and send us as per your earliest convenience. It will be a great help if could send the protocol exactly as used in experiment.

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